CAS 53643-14-4

4-methylumbelliferyl N,N',N'',N'''-*tetraacetyl-B

Description:

4-Methylumbelliferyl N,N',N'',N'''-tetraacetyl-B, identified by its CAS number 53643-14-4, is a synthetic compound commonly used as a fluorogenic substrate in biochemical assays. This compound features a 4-methylumbelliferone moiety, which is known for its fluorescent properties, making it useful in detecting enzymatic activity. The tetraacetyl groups enhance its stability and solubility, allowing for better performance in various applications, particularly in the study of enzyme kinetics and activity. Upon enzymatic cleavage, the compound releases a fluorescent product, which can be quantitatively measured, providing insights into the enzymatic processes being studied. Its characteristics include a relatively low molecular weight and a specific excitation and emission spectrum, which are essential for fluorescence-based detection methods. Additionally, it is important to handle this compound with care, following appropriate safety protocols, as with all chemical substances, to mitigate any potential hazards associated with its use in laboratory settings.

Formula: C₄₂H₆₀N₄O₂₃

InChI: InChI=1/C42H60N4O23/c1-14-8-26(55)62-21-9-19(6-7-20(14)21)61-39-28(44-16(3)52)33(58)36(23(11-48)64-39)68-41-30(46-18(5)54)35(60)38(25(13-50)66-41)69-42-29(45-17(4)53)34(59)37(24(12-49)65-42)67-40-27(43-15(2)51)32(57)31(56)22(10-47)63-40/h6-9,22-25,27-42,47-50,56-60H,10-13H2,1-5H3,(H,43,51)(H,44,52)(H,45,53)(H,46,54)

SMILES: Cc1cc(=O)oc2cc(ccc12)OC1C(C(C(C(CO)O1)OC1C(C(C(C(CO)O1)OC1C(C(C(C(CO)O1)OC1C(C(C(C(CO)O1)O)O)N=C(C)O)O)N=C(C)O)O)N=C(C)O)O)N=C(C)O

Synonyms:

• 4-Methylumbelliferyl b-D-N,N,N,N-tetraacetylchitotetraoside
• 4-methyl-2-oxo-2H-chromen-7-yl 2-(acetylamino)-2-deoxyhexopyranosyl-(1->4)-2-(acetylamino)-2-deoxyhexopyranosyl-(1->4)-2-(acetylamino)-2-deoxyhexopyranosyl-(1->4)-2-(acetylamino)-2-deoxyhexopyranoside

4-Methylumbelliferyl N,N'',N'''',N''''''-tetraacetyl-b-D-chitotetraoside

CAS: 53643-14-4

Ref: 7W-GC9001

4-Methylumbelliferyl N,N',N'',N'''-tetraacetyl-b-D-chitotetraoside

CAS: 53643-14-4

4-Methylumbelliferyl N,N',N'',N'''-tetraacetyl-beta-D-chitotetraoside is a fluorogenic substrate for chitinase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl N,N',N'',N'''-tetraacetyl-beta-D-chitotetraosideas a substrate for measuring the chitinase activity is used for fungal infection detection and Gaucher disease screening.

Formula: C₄₂H₆₀N₄O₂₃

Purity: Min. 95%

Color and Shape: Powder

Molecular weight: 988.94 g/mol

Ref: 3D-EM04831