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CAS 54827-17-7

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3,3′,5,5′-Tetramethylbenzidine

Description:
3,3′,5,5′-Tetramethylbenzidine (TMB) is an organic compound commonly used as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISA) and other biochemical applications. It is characterized by its ability to undergo oxidation, resulting in a color change that can be quantitatively measured, making it valuable in laboratory diagnostics. TMB is a derivative of benzidine, featuring four methyl groups attached to the benzene rings, which enhances its solubility and stability. The compound is typically a pale yellow to colorless solid at room temperature and is soluble in organic solvents and water under certain conditions. TMB is sensitive to light and air, which can lead to degradation; therefore, it is often stored in dark, airtight containers. While TMB is widely used in research and clinical settings, it is important to handle it with care due to its potential toxicity and carcinogenic properties associated with its benzidine structure. Proper safety protocols should be followed when working with this compound to minimize exposure risks.
Formula:C16H20N2
InChI:InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key:InChIKey=UAIUNKRWKOVEES-UHFFFAOYSA-N
SMILES:CC=1C=C(C=C(C)C1N)C2=CC(C)=C(N)C(C)=C2
Synonyms:
  • 1'-Biphenyl)-4,4'-Diamine,3,3',5,5'-Tetramethyl-(
  • 1'-Biphenyl]-4,4'-Diamine,3,3',5,5'-Tetramethyl-[1
  • 3,3',5,5'-Tetramethyl benzidine
  • 3,3',5,5'-Tetramethylbenzidin
  • 3,3',5,5'-Tetramethylbiphenyl-4,4'-Diamine
  • 3,3',5,5'-Tetrametilbencidina
  • 3,3,5,5-Tetramethylbenzidine
  • 3,3′,5,5′-Tetramethyl-4,4′-diaminobiphenyl
  • 3,3′,5,5′-Tetramethyl[1,1′-biphenyl]-4,4′-diamine
  • 3,3′,5,5′-Tetramethylbiphenyl-4,4′-diamine
  • See more synonyms
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