CAS 54827-17-7: 3,3′,5,5′-Tetramethylbenzidine

Description: 3,3′,5,5′-Tetramethylbenzidine (TMB) is an organic compound commonly used as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISA) and other biochemical applications. It is characterized by its ability to undergo oxidation, resulting in a color change that can be quantitatively measured, making it valuable in laboratory diagnostics. TMB is a derivative of benzidine, featuring four methyl groups attached to the benzene rings, which enhances its solubility and stability. The compound is typically a pale yellow to colorless solid at room temperature and is soluble in organic solvents and water under certain conditions. TMB is sensitive to light and air, which can lead to degradation; therefore, it is often stored in dark, airtight containers. While TMB is widely used in research and clinical settings, it is important to handle it with care due to its potential toxicity and carcinogenic properties associated with its benzidine structure. Proper safety protocols should be followed when working with this compound to minimize exposure risks.

Formula: C₁₆H₂₀N₂

InChI: InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key: InChIKey=UAIUNKRWKOVEES-UHFFFAOYSA-N

SMILES: NC=1C(=CC(=CC1C)C=2C=C(C(N)=C(C2)C)C)C

Synonyms:

• 1'-Biphenyl)-4,4'-Diamine,3,3',5,5'-Tetramethyl-(
• 1'-Biphenyl]-4,4'-Diamine,3,3',5,5'-Tetramethyl-[1
• 3,3',5,5'-Tetramethyl benzidine
• 3,3',5,5'-Tetramethylbenzidin
• 3,3',5,5'-Tetramethylbiphenyl-4,4'-Diamine
• 3,3',5,5'-Tetrametilbencidina
• 3,3,5,5-Tetramethylbenzidine
• 3,3′,5,5′-Tetramethyl-4,4′-diaminobiphenyl
• 3,3′,5,5′-Tetramethyl[1,1′-biphenyl]-4,4′-diamine
• 3,3′,5,5′-Tetramethylbiphenyl-4,4′-diamine
• 3,5,3',5'-Tetramethylbenzidine
• 3,5,3′,5′-Tetramethylbenzidine
• 3355Tmb
• 4,4'-Bi-2,6-Xylidine
• 4,4'-Diamino-3,3',5,5'-Tetramethylbiphenyl
• BM blue
• Benzidine, 3,3',5,5'-tetramethyl-
• Benzidine, 3,3′,5,5′-tetramethyl-
• ColorBurst Blue
• Enhanced K-Blue
• K-Blue
• K-Blue Max
• N-(3,5-dimethylphenyl)-3,5-dimethylaniline
• Potassium-Blue Max
• Sure Blue TMB
• TMB
• TMB Blotting Plus
• TMB substrate
• Tetramethylbenzidine
• Tmb Quick
• [1,1'-Biphenyl]-4,4'-diamine, 3,3',5,5'-tetramethyl-
• [1,1′-Biphenyl]-4,4′-diamine, 3,3′,5,5′-tetramethyl-

3,3',5,5'-Tetramethylbenzidine [for Biochemical Research]

CAS: 54827-17-7

• Formula: C₁₆H₂₀N₂
• Purity: >98.0%(T)(HPLC)
• Color and Shape: White to Green to Brown powder to crystaline
• Molecular weight: 240.35

3,3',5,5'-Tetramethylbenzidine, 98%

CAS: 54827-17-7

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes; particularly useful in ELISA techniques, …

• Formula: C₁₆H₂₀N₂
• Purity: 98%
• Color and Shape: Powder, White to cream to pale brown
• Molecular weight: 240.35

3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, precipitating, high sensitivity

CAS: 54827-17-7

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is used with peroxidase …

• Formula: C₁₆H₂₀N₂
• Color and Shape: Colorless, Liquid
• Molecular weight: 240.35

3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity

CAS: 54827-17-7

TMB is a substrate for horseradish peroxidase. Recommended for ELISA (microwell) procedures,3,3?,5,5?-Tetramethylbenzidine has been used …

• Formula: C₁₆H₂₀N₂
• Color and Shape: Clear colorless to pale blue, Liquid
• Molecular weight: 240.35

3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, standard sensitivity

CAS: 54827-17-7

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is used with peroxidase …

• Formula: C₁₆H₂₀N₂
• Color and Shape: Clear liquid
• Molecular weight: 240.35

3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, precipitating, standard sensitivity

CAS: 54827-17-7

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is used with peroxidase …

• Formula: C₁₆H₂₀N₂
• Color and Shape: Colorless, Liquid
• Molecular weight: 240.35

3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine

CAS: 54827-17-7

• Formula: C₁₆H₂₀N₂
• Purity: 98%
• Color and Shape: Solid
• Molecular weight: 240.3434

3,3',5,5'-Tetramethylbiphenyl-4,4'-diamine

CAS: 54827-17-7

• Formula: C₁₆H₂₀N₂
• Purity: By gc: 99.8% by area (Typical Value in Batch COA)
• Color and Shape: faint yellow powder
• Molecular weight: 240.34g/mol

3,3',5,5'-Tetramethylbenzidine, free base

CAS: 54827-17-7

• Color and Shape: Powder
• Molecular weight: 240.34g/mol

TMB

CAS: 54827-17-7

• Purity: 99.96%
• Molecular weight: 240.34g/mol

3,3'',5,5''-Tetramethylbenzidine

CAS: 54827-17-7

• Formula: C₁₆H₂₀N₂
• Purity: ≥ 98.0% (dried basis)
• Color and Shape: White to light-beige or pale yellow crystalline
• Molecular weight: 240.35

3,3',5,5'-Tetramethylbenzidine, 99+%

CAS: 54827-17-7

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. …

• Formula: C₁₆H₂₀N₂
• Purity: 99+%
• Color and Shape: White to cream to light yellow, Crystalline powder
• Molecular weight: 240.35

3,3',5,5'-Tetramethylbenzidine

CAS: 54827-17-7

• Formula: C₁₆H₂₀N₂
• Color and Shape: Neat
• Molecular weight: 240.34

3,3',5,5'-Tetramethylbenzidine

CAS: 54827-17-7

• Formula: C₁₆H₂₀N₂
• Purity: >98.0%(T)(HPLC)
• Color and Shape: White to Gray to Brown powder to crystal
• Molecular weight: 240.35

TMB

CAS: 54827-17-7

TMB (BM blue) is a chromogenic substrate. It used in staining procedures in immunohistochemistry and …

• Formula: C₁₆H₂₀N₂
• Purity: 100% - 99.96%
• Color and Shape: Physical Description Pale Yellow Crystals Or Off-White Powder (Ntp 1992)
• Molecular weight: 240.34

3,3',5,5'-Tetramethylbenzidine

CAS: 54827-17-7

Applications 3,3',5,5'-Tetramethylbenzidine is a chromogenic substrate used in staining procedures in immunohistochemistry as well as …

• Formula: C₁₆H₂₀N₂
• Color and Shape: Neat
• Molecular weight: 240.34

3,3',5,5'-Tetramethylbenzidine, free base

CAS: 54827-17-7

TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is …

• Formula: C₁₆H₂₀N₂
• Purity: Min. 99.0 Area-%
• Molecular weight: 240.35 g/mol

3,3',5,5'-Tetramethylbenzidine, free base

CAS: 54827-17-7

Please enquire for more information about 3,3',5,5'-Tetramethylbenzidine, free base including the price, delivery time and …

• Formula: C₁₆H₂₀N₂
• Purity: Min. 99.0 Area-%
• Molecular weight: 240.35 g/mol

3,3',5,5'-Tetramethylbenzidine, free base

CAS: 54827-17-7

Please enquire for more information about 3,3',5,5'-Tetramethylbenzidine, free base including the price, delivery time and …

• Formula: C₁₆H₂₀N₂
• Molecular weight: 240.35 g/mol

3,3,5,5-Tetramethylbenzidine (TMB) extrapure AR, ExiPlus, Multi-Compendial, 98%

CAS: 54827-17-7

• Formula: C₁₆H₂₀N₂
• Purity: min. 98.0%
• Color and Shape: Off - white to brown, Crystalline powder
• Molecular weight: 240.35