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CAS 55699-64-4

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Benzothiazolyldiphenyltetrazoliumbromide

Description:
Benzothiazolyldiphenyltetrazoliumbromide, commonly referred to as MTT, is a yellow, water-soluble compound widely used in biological and biochemical assays, particularly for cell viability and proliferation studies. Its structure features a benzothiazole moiety linked to diphenyl and tetrazolium groups, which contribute to its unique properties. Upon reduction by metabolically active cells, MTT is converted into a purple formazan product, allowing for quantification of cellular activity through spectrophotometric measurement. This compound is non-toxic to cells at low concentrations, making it suitable for in vitro studies. MTT is sensitive to light and should be stored in a cool, dark place to maintain stability. Its applications extend beyond cell viability assays to include studies on mitochondrial function and cytotoxicity testing. Overall, MTT serves as a valuable tool in research for assessing cell health and metabolic activity, providing insights into various biological processes.
Formula:C20H14BrN5S
InChI:InChI=1/C20H14N5S.BrH/c1-3-9-15(10-4-1)19-22-24(16-11-5-2-6-12-16)25(23-19)20-21-17-13-7-8-14-18(17)26-20;/h1-14H;1H/q+1;/p-1
Synonyms:
  • 2-(2-Benzothiazolyl)-3,5-diphenyltetrazolium bromide
  • 3-(1,3-benzothiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide
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