CAS 5725-91-7

7-Ethoxyresorufin

Description:

7-Ethoxyresorufin is a synthetic organic compound commonly used as a substrate in biochemical assays, particularly for measuring the activity of cytochrome P450 enzymes. It is characterized by its structure, which includes an ethoxy group and a resorufin moiety, contributing to its fluorescent properties. This compound is typically utilized in toxicological studies and drug metabolism research due to its ability to be converted into a highly fluorescent product upon enzymatic action. The fluorescence emitted upon conversion allows for sensitive detection and quantification, making it a valuable tool in pharmacokinetics and environmental monitoring. 7-Ethoxyresorufin is soluble in organic solvents and exhibits stability under standard laboratory conditions. Its use in assays is often accompanied by considerations of substrate specificity and enzyme kinetics, as different cytochrome P450 isoforms may exhibit varying levels of activity towards this substrate. Overall, 7-Ethoxyresorufin serves as an important reagent in the study of metabolic processes and enzyme function.

Formula: C₁₄H₁₁NO₃

InChI: InChI=1S/C14H11NO3/c1-2-17-10-4-6-12-14(8-10)18-13-7-9(16)3-5-11(13)15-12/h3-8H,2H2,1H3

InChI key: InChIKey=CRCWUBLTFGOMDD-UHFFFAOYSA-N

SMILES: O(CC)C=1C=C2C(N=C3C(O2)=CC(=O)C=C3)=CC1

Synonyms:

• 3H-Phenoxazin-3-one, 7-ethoxy-
• 7-Ethoxyphenoxazin-3-one
• 7-Ethoxyphenoxazone
• 7-Ethoxyresorufin
• 7-ethoxy-3H-phenoxazin-3-one
• O-Ethylresorufin
• Resorufin ethyl ether
• Resorufin ethyl ether (Ethoxyresorufin)

7-Ethoxyresorufin

CAS: 5725-91-7

Fluorimetric substrate for detection of cytochrome P450 linked enzymes

Formula: C₁₄H₁₁NO₃

Molecular weight: 241.24

Ref: 02-J62987

7-Ethoxy-3H-phenoxazin-3-one

CAS: 5725-91-7

Formula: C₁₄H₁₁NO₃

Purity: 98%

Color and Shape: Solid

Molecular weight: 241.2420

Ref: IN-DA0038MP

7-Ethoxyresorufin

CAS: 5725-91-7

7-Ethoxyresorufin

Purity: ≥98%

Molecular weight: 241.24g/mol

Ref: 54-BUP01918

Ethoxy Resorufin

CAS: 5725-91-7

Formula: C₁₄H₁₁NO₃

Color and Shape: Red Solid

Molecular weight: 241.25

Ref: 4Z-R-253

Resorufin ethyl ether

CAS: 5725-91-7

Formula: C₁₄H₁₁NO₃

Purity: (UV) ≥ 95.0%

Color and Shape: Orange to red solid

Molecular weight: 241.24

Ref: 7W-GL6291

7-Ethoxyresorufin

CAS: 5725-91-7

7-Ethoxyresorufin (Resorufin ethyl ether) (7-ER) is a fluorometric substrate for and competitive inhibitor of cytochrome P450, especially CYP1A1.

Formula: C₁₄H₁₁NO₃

Purity: 99.09% - 99.62%

Color and Shape: Red Solid

Molecular weight: 241.24

Ref: TM-T21507

7-Ethoxy Resorufin

Controlled Product

CAS: 5725-91-7

Applications A fluorimetric substrate for cytochrome P450 IAI. Inhibits vasorelaxant responses to acetylcholine.
References Donato, M.T., et al.: Anal. Biochem., 213, 29 (1993), Oyekan, A.O., et al.: J. Pharmacol. Exp. Ther., 268, 262 (1994)

Formula: C₁₄H₁₁NO₃

Color and Shape: Orange To Dark Red

Molecular weight: 241.24

Ref: TR-E892875

Resorufin ethyl ether

CAS: 5725-91-7

Resorufin ethyl ether is a fluorescent substrate that is used to measure the activity of cytochrome P450 enzymes. It can be used in vitro to study the effects of drugs on enzyme activities and in vivo to study liver lesions. Resorufin ethyl ether is also used as a model system for studying synchronous fluorescence, which is the emission of light by two or more substances at the same time. The nitrogen atoms in resorufin ethyl ether can be substituted with other atoms such as magnesium or zinc, which will affect its activity and fluorescence properties. Resorufin ethyl ether binds to cytochrome P450 enzymes and therefore inhibits their activity. This inhibition is reversible, and it can be reversed by adding a substance that competes with the substrate for binding sites on the enzyme.

Purity: Min. 95%

Molecular weight: 241.24 g/mol

Ref: 3D-ER22912