CAS 596-09-8
:Fluorescein, diacetate
Description:
Fluorescein diacetate (CAS 596-09-8) is a non-fluorescent, lipophilic compound that serves as a vital cell-permeable probe in biological research. Upon entering cells, it is hydrolyzed by intracellular esterases to produce fluorescein, a highly fluorescent compound. This property makes fluorescein diacetate an effective tool for assessing cell viability and membrane integrity, as only live cells can convert it into its fluorescent form. The substance is typically characterized by its bright green fluorescence under ultraviolet light, which allows for easy visualization in various applications, including microscopy and flow cytometry. Fluorescein diacetate is soluble in organic solvents and has a relatively low molecular weight, contributing to its ability to diffuse across cell membranes. Its stability and ease of use make it a popular choice in studies involving live-cell imaging, apoptosis, and cytotoxicity assays. However, proper handling and storage conditions are essential to maintain its efficacy and prevent degradation.
Formula:C24H16O7
InChI:InChI=1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
InChI key:InChIKey=CHADEQDQBURGHL-UHFFFAOYSA-N
SMILES:O=C1OC2(C=3C(OC=4C2=CC=C(OC(C)=O)C4)=CC(OC(C)=O)=CC3)C=5C1=CC=CC5
Synonyms:- (6′-Acetyloxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-3′-yl) acetate
- 3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate
- 3′,6′-Bis(acetyloxy)spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
- 3′,6′-Diacetylfluorescein
- Diacetylfluorescein
- F 1303
- Fluorescein 3′,6′-diacetate
- Fluorescein Diacetat
- Mfcd 5062
- NSC 4726
- NSC 667259
- Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, 3′,6′-bis(acetyloxy)-
- Fluorescein, diacetate
- See more synonyms
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Fluorescein Diacetate
CAS:Formula:C24H16O7Purity:>98.0%(HPLC)Color and Shape:White to Light yellow powder to crystalineMolecular weight:416.39Diacetylfluorescein
CAS:DiacetylfluoresceinFormula:anhydrousdiacetylfluoresceinPurity:97.7%Color and Shape:Light Yellow. To. OrangeDiacetylfluorescein
CAS:Aromatic heterocyclic pesticides with oxygen hetero-atom(s) only, nesoiFormula:C24H16O7Color and Shape:Pale Yellow Tan PowderMolecular weight:416.0896Fluorescein diacetate
CAS:Fluorescein diacetateFormula:C24H16O7Purity:By hplc: >98% (Typical Value in Batch COA)Color and Shape:Light yellow Solid-PowderMolecular weight:416.37964Diacetylfluorescein
CAS:Controlled ProductFormula:C24H16O7Color and Shape:NeatMolecular weight:416.383-Oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate
CAS:Formula:C24H16O7Purity:97%Color and Shape:SolidMolecular weight:416.3796Diacetylfluorescein
CAS:Controlled ProductApplications Diacetylfluorescein is used in fluorometric staining in the imaging of mammalian cells. Also used in the synthesis of nanocrystal biolabels with releasable fluorophores used in immunoassays. Dyes and metabolites.
References Chan, C. et al.: Anal. Chem., 76, 3638 (2004); Chan, C. et al.: Anal. Chem., 76, 3638 (2004);Formula:C24H16O7Color and Shape:NeatMolecular weight:416.38Fluorescein diacetate
CAS:Fluorescein diacetate (Di-O-acetylfluorescein) is a cell permeable esterase-substrate for hGSTP1-1.Formula:C24H16O7Purity:99.61% - 99.79%Color and Shape:White SolidMolecular weight:416.38Fluorescein diacetate
CAS:Formula:C24H16O7Purity:≥ 97.0%Color and Shape:White to yellow crystalline powderMolecular weight:416.39Fluorescein diacetate
CAS:Fluorogenic substrate targeting esterases , hGSTP1-1
Formula:C24H16O7Color and Shape:PowderMolecular weight:416.38 g/molFluorescein Diacetate
CAS:3-Oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetateFormula:C24H16O7Purity:98%Molecular weight:416.37Fluorescein di-O-acetate (FDA)
CAS:M03592 - Fluorescein di-O-acetate (FDA)
Formula:C24H16O7Purity:98%Color and Shape:SolidMolecular weight:416.385
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