N-Cbz-ala-ala-leu P-nitroanilide, with the CAS number 61043-33-2, is a synthetic peptide derivative commonly used in biochemical research, particularly in the study of proteolytic enzymes. This compound features a Cbz (carbobenzyloxy) protecting group, which is often employed to stabilize the amino acid residues during synthesis and manipulation. The structure includes two alanine (ala) residues and one leucine (leu) residue, contributing to its peptide characteristics. The P-nitroanilide moiety serves as a chromogenic or fluorogenic tag, allowing for the monitoring of enzymatic activity through changes in absorbance or fluorescence. This compound is typically utilized in assays to evaluate the activity of serine proteases and other enzymes that cleave peptide bonds. Its stability, solubility in organic solvents, and the ability to undergo specific enzymatic cleavage make it a valuable tool in biochemical and pharmaceutical research. As with many peptide derivatives, proper handling and storage conditions are essential to maintain its integrity and functionality.

N-cbz-ala-ala-leu P-nitroanilide

61043-33-2: N-Cbz-ala-ala-leu P-nitroanilide, with the CAS number 61043-33-2, is a synthetic peptide derivative commonly used in biochemical research, particularly in the study of proteolytic enzymes. This compound features a Cbz (carbobenzyloxy) protecting group, which is often employed to stabilize the amino acid residues during synthesis and manipulation. The structure includes two alanine (ala) residues and one leucine (leu) residue, contributing to its peptide characteristics. The P-nitroanilide moiety serves as a chromogenic or fluorogenic tag, allowing for the monitoring of enzymatic activity through changes in absorbance or fluorescence. This compound is typically utilized in assays to evaluate the activity of serine proteases and other enzymes that cleave peptide bonds. Its stability, solubility in organic solvents, and the ability to undergo specific enzymatic cleavage make it a valuable tool in biochemical and pharmaceutical research. As with many peptide derivatives, proper handling and storage conditions are essential to maintain its integrity and functionality.

<p>Z-Ala-Ala-Leu-pNA is a synthetic substrate that is used as a chemical test for serine proteases. It is synthesized by the addition of a single amino acid to the C-terminal end of Z-Ala-Ala-Leu. This substrate has been used in kinetic studies and biochemical analysis, such as biophysical and proteolytic properties. The enzyme preparation that hydrolyzes this substrate is often immobilized on a solid support, such as agarose beads or polystyrene or glass beads, so that it can be applied to an assay plate. Z-Ala-Ala-Leu-pNA is also used in functional group analyses of proteins.</p>

CAS 61043-33-2

N-cbz-ala-ala-leu P-nitroanilide

Z-Ala-Ala-Leu-pNA

Ref: 3D-SAP-3127