Ala-Ala-Phe P-nitroanilide, with the CAS number 61043-41-2, is a synthetic peptide substrate commonly used in biochemical assays, particularly in the study of proteolytic enzymes. This compound consists of a sequence of amino acids, specifically two alanine residues followed by a phenylalanine residue, linked to a p-nitroanilide moiety. The p-nitroanilide group serves as a chromogenic marker, allowing for the detection of enzymatic activity through colorimetric changes. The characteristics of this substance include its solubility in organic solvents and water, stability under standard laboratory conditions, and its role as a substrate in enzyme kinetics studies. It is often employed in assays to measure the activity of serine proteases and other enzymes that cleave peptide bonds. Safety data should be consulted, as with any chemical, to ensure proper handling and disposal. Overall, Ala-Ala-Phe P-nitroanilide is a valuable tool in biochemical research for understanding enzyme mechanisms and kinetics.

ala-ala-phe P-nitroanilide

61043-41-2: Ala-Ala-Phe P-nitroanilide, with the CAS number 61043-41-2, is a synthetic peptide substrate commonly used in biochemical assays, particularly in the study of proteolytic enzymes. This compound consists of a sequence of amino acids, specifically two alanine residues followed by a phenylalanine residue, linked to a p-nitroanilide moiety. The p-nitroanilide group serves as a chromogenic marker, allowing for the detection of enzymatic activity through colorimetric changes. The characteristics of this substance include its solubility in organic solvents and water, stability under standard laboratory conditions, and its role as a substrate in enzyme kinetics studies. It is often employed in assays to measure the activity of serine proteases and other enzymes that cleave peptide bonds. Safety data should be consulted, as with any chemical, to ensure proper handling and disposal. Overall, Ala-Ala-Phe P-nitroanilide is a valuable tool in biochemical research for understanding enzyme mechanisms and kinetics.

Substrate for chymotrypsin and tripeptidyl peptidases I and II.

CAS 61043-41-2

ala-ala-phe P-nitroanilide

H-Ala-Ala-Phe-pNA

Ref: 01-4002753

Ala-Ala-Phe p-nitroanilide

Ref: IN-DA00EC6Z