Z-Ala-Ala-pNA, also known as Z-Ala-Ala-p-nitroanilide, is a synthetic peptide derivative commonly used in biochemical research, particularly in enzyme assays. The "Z" in its name indicates that it is a protected form of the amino acid alanine, where the N-terminal amino group is protected by a benzyloxycarbonyl (Z) group. This protection allows for stability during synthesis and manipulation in laboratory settings. The compound features two alanine residues linked by peptide bonds, and the pNA (p-nitroanilide) moiety serves as a chromogenic substrate, which can be cleaved by certain proteolytic enzymes, releasing p-nitroaniline and allowing for the quantification of enzymatic activity. Z-Ala-Ala-pNA is characterized by its ability to undergo hydrolysis in the presence of specific enzymes, making it a valuable tool for studying protease activity. Its molecular structure and properties make it suitable for various applications in peptide synthesis and enzyme kinetics.

Z-Ala-Ala-pNA

61043-58-1: Z-Ala-Ala-pNA, also known as Z-Ala-Ala-p-nitroanilide, is a synthetic peptide derivative commonly used in biochemical research, particularly in enzyme assays. The "Z" in its name indicates that it is a protected form of the amino acid alanine, where the N-terminal amino group is protected by a benzyloxycarbonyl (Z) group. This protection allows for stability during synthesis and manipulation in laboratory settings. The compound features two alanine residues linked by peptide bonds, and the pNA (p-nitroanilide) moiety serves as a chromogenic substrate, which can be cleaved by certain proteolytic enzymes, releasing p-nitroaniline and allowing for the quantification of enzymatic activity. Z-Ala-Ala-pNA is characterized by its ability to undergo hydrolysis in the presence of specific enzymes, making it a valuable tool for studying protease activity. Its molecular structure and properties make it suitable for various applications in peptide synthesis and enzyme kinetics.

<p>Z-Ala-Ala-pNA is a bifunctional endopeptidase that has regulatory and subtilisin activity. It is used to regulate the production of nitrate in microorganisms such as bacteria and fungi, which can be an important factor in the production of food products. Z-Ala-Ala-pNA is a recombinant enzyme that was developed from a strain of Bacillus subtilis. This enzyme has binding sites for both serine proteinases and sodium nitrate. It degrades proteins by cleaving them at their serine residues, releasing amino acids and peptides from the N-terminus of the protein. The regulatory function of this enzyme is due to its ability to bind to nitrate ions, thereby regulating their concentration in cells.</p>

CAS 61043-58-1

Z-Ala-Ala-pNA

Z-Ala-Ala-pNA

Ref: 3D-FA110750