Ac-Ala-Pro-Ala-pNA, also known as N-acetyl-L-alanyl-L-prolyl-L-alanylp-nitroanilide, is a synthetic peptide substrate commonly used in biochemical research, particularly in studies involving proteolytic enzymes. This compound features a sequence of amino acids, specifically N-acetylated alanine and proline, linked to a p-nitroanilide moiety, which serves as a chromogenic group. The presence of the p-nitroanilide allows for colorimetric detection upon cleavage by specific proteases, making it valuable for enzyme activity assays. The peptide's structure contributes to its stability and specificity, as the sequence can influence the binding and recognition by enzymes. Additionally, Ac-Ala-Pro-Ala-pNA is soluble in various organic solvents and aqueous solutions, facilitating its use in laboratory settings. Its molecular weight and specific functional groups also play a role in its reactivity and interactions with other biomolecules. Overall, Ac-Ala-Pro-Ala-pNA is a useful tool in the study of enzymatic processes and peptide chemistry.

Ac-Ala-Pro-Ala-pNA

61596-39-2: Ac-Ala-Pro-Ala-pNA, also known as N-acetyl-L-alanyl-L-prolyl-L-alanylp-nitroanilide, is a synthetic peptide substrate commonly used in biochemical research, particularly in studies involving proteolytic enzymes. This compound features a sequence of amino acids, specifically N-acetylated alanine and proline, linked to a p-nitroanilide moiety, which serves as a chromogenic group. The presence of the p-nitroanilide allows for colorimetric detection upon cleavage by specific proteases, making it valuable for enzyme activity assays. The peptide's structure contributes to its stability and specificity, as the sequence can influence the binding and recognition by enzymes. Additionally, Ac-Ala-Pro-Ala-pNA is soluble in various organic solvents and aqueous solutions, facilitating its use in laboratory settings. Its molecular weight and specific functional groups also play a role in its reactivity and interactions with other biomolecules. Overall, Ac-Ala-Pro-Ala-pNA is a useful tool in the study of enzymatic processes and peptide chemistry.

<p>Ac-Ala-Pro-Ala-pNA is a transition-state analog inhibitor of serine proteases. In the catalytic cleft, Ac-Ala-Pro-Ala-pNA mimics the carbonyl group and minimizes the active site serine. The hydroxyl group in this compound is responsible for its transition state buildup that leads to hydrolytic reactions. Acetal formation can be observed as an intermediate step in this reaction. Acetal formation occurs when a hydroxylic oxygen atom reacts with a terminal alkoxy or thiohydroxylic carbon atom on the reactant molecule. This reaction is catalyzed by enzymes such as alcohol dehydrogenase, acetaldehyde dehydrogenase, and acetyl transferase.</p>

CAS 61596-39-2

Ac-Ala-Pro-Ala-pNA

Ac-Ala-Pro-Ala-pNA

Ref: 3D-FA110693