CAS 61970-00-1

Firefly luciferase

Description:

Firefly luciferase is an enzyme derived from fireflies, specifically from the species Photinus pyralis, and is known for its role in bioluminescence. This enzyme catalyzes the oxidation of luciferin, a light-emitting compound, in the presence of oxygen, resulting in the production of light. Firefly luciferase is characterized by its high specificity for luciferin and its ability to produce light in a range of conditions, making it a valuable tool in molecular biology and biochemistry. It is commonly used in bioluminescent assays, gene expression studies, and as a reporter gene in various applications due to its sensitivity and ease of detection. The enzyme operates optimally at physiological pH and temperature, and its activity can be influenced by factors such as ionic strength and the presence of cofactors. The CAS number 61970-00-1 uniquely identifies this enzyme in chemical databases, facilitating research and application in scientific studies. Overall, firefly luciferase is a crucial component in various biotechnological applications, leveraging its natural luminescent properties.

Formula: Unspecified

Synonyms:

• Beetle luciferase
• Click beetle luciferase
• E.C. 1.13.12.7
• Ec 1.13.12.7
• Firefly luciferase
• Firefly luciferin luciferase
• Luciferasa (Luciferina De Luciernaga)
• Luciferase
• Luciferase (Leuchtkaferluciferin)
• Luciferase (Luciferine De Luciole)
• Luciferase (Macrolampis luciferin)
• Luciferase (firefly)
• Luciferase-LM plus
• Luciola mingrelica luciferase
• Macrolampis luciferase
• Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing)
• Photinus pyralis luciferase
• Photinus scintillans luciferase
• Promega luciferase
• Pyrocoelia pygidialis luciferase
• Pyrophorus plagiophthalamus luciferase
• QuantiLum Recombinant Luciferase
• RFLuc
• Ultra-Glo
• Ultra-Glo luciferase
• Luciferase, Firefly, Recomb.
• Luciferase (firefly luciferin)

Luciferase, firefly

CAS: 61970-00-1

Luciferase, firefly is a reporter gene in cellular imaging studies to quantify target gene expression levels and measure intracellular ATP content,

Purity: 98%

Color and Shape: Solid

Molecular weight: N/A

Ref: TM-TP1774

X-Shining™ Luciferase, lyophilised

CAS: 61970-00-1

Supplied as lyophilisate containing 1% w/w X-Shining™ Luciferase. The improved, thermostable X-Shining™ Luciferase is suitable for any luciferin-luciferase-based assay using D-luciferin (dLuc) or synthetic pro-luciferins (caged luciferins). Examples of its typical applications include ATP tests in hygiene monitoring, ATP tests in drug screenings and bacteria identification in microbial assays. The termostable luciferase X-Shining™ has been optimised by genetic engineering for strongly increased thermostability and storage stability. In temperature stress tests, the enzyme survives temperatures of 60°C for over an hour, whereas a wild type luciferase from firefly is inactivated after only a few minutes. The extraordinary stability makes it user-friendly and eliminates some of the main disadvantages and limitations of the commonly used wild type luciferase. X-Shining™ Luciferase is supplied as a lyophilisate (1% w/w X-Shining™ Luciferase) or as aqueous solution with glycerol (L-8093), and it may be stored for months at room temperature without significant loss of function. Find out more about our innovative X-Shining range here X-Shining | Innovation | Cymit Quimica Carbosynth.

Ref: 3D-L-8095

Luciferase - from Photinus pyralis (firefly)

CAS: 61970-00-1

Luciferase enzyme from Photinus pyralis (firefly), which catalyzes the oxidation of firefly luciferin. This reaction depends on the presence of oxygen and ATP and causes the bioluminescence seen in fireflies

Purity: (Gel Electrophoresis) Min. 98%

Color and Shape: Powder

Ref: 3D-FL162293

X-Shining™ Luciferase, 10 mg/mL aqueous solution with glycerol

CAS: 61970-00-1

The improved, thermostable X-ShiningTM Luciferase is suitable for any luciferin-luciferase-based assay using D-luciferin (dLuc) or synthetic pro-luciferins (caged luciferins). Examples of its typical applications include ATP tests in hygiene monitoring, ATP tests in drug screenings and bacteria identification in microbial assays. The thermostable luciferase X-ShiningTM has been optimised by genetic engineering for strongly increased thermostability and storage stability. In temperature stress tests, the enzyme survives temperatures of 60 °C for over an hour, whereas a wild type luciferase from firefly is inactivated after only a few minutes. The extraordinary stability makes it user-friendly and eliminates some of the main disadvantages and limitations of the commonly used wild type luciferase. X-ShiningTM Luciferase is supplied as aqueous solution with glycerol or as a lyophilisate (L-8095), and it may be stored for months at room temperature without significant loss of function. Find out more about our innovative X-Shining range here X-Shining | Innovation | Cymit Quimica Carbosynth.

Purity: (Sds-Page) Min. 90%

Ref: 3D-L-8093

econoLuciferase, buffered aqueous solution

CAS: 61970-00-1

Cymit Quimica’s econoLuciferase™ (econoLuc Cymit Quimica Cat. No. L-8090) is a recombinant luciferase from the firefly Luciola lateralis that has been expressed as a luciferase-GFP fusion protein in E. coli.The luciferase enzyme has been optimized for increased thermo-stability by genetic modification to be stable for one hour at 37°C and up to two days at room temperature.Stabilized econoLuciferase™ thus overcomes the disadvantages and limitations of wild-type Luciferase, notably its short active life, outperforming enzyme from the Photinus pyralis firefly for both performance and stability.This optimized luciferase is the perfect enzyme for ATP detection assays in hygiene control, microbial tests using caged luciferins and it is the luciferase of choice for biochemical tests measuring ATP consumption and production in diverse enzymatic reactions.The product L-8090 is available on a large scale and is intended for use in the chemical, diagnostic, pharmaceutical and related industries.

Purity: (Spec. Activity. U/Mg) Min. 5 X 10^8

Ref: 3D-L-8090

X-Shining™ Luciferase, lyophilised

CAS: 61970-00-1

Supplied as lyophilisate containing 1% w/w X-Shining™ Luciferase. The improved, thermostable X-Shining™ Luciferase is suitable for any luciferin-luciferase-based assay using D-luciferin (dLuc) or synthetic pro-luciferins (caged luciferins). Examples of its typical applications include ATP tests in hygiene monitoring, ATP tests in drug screenings and bacteria identification in microbial assays. The termostable luciferase X-Shining™ has been optimised by genetic engineering for strongly increased thermostability and storage stability. In temperature stress tests, the enzyme survives temperatures of 60°C for over an hour, whereas a wild type luciferase from firefly is inactivated after only a few minutes. The extraordinary stability makes it user-friendly and eliminates some of the main disadvantages and limitations of the commonly used wild type luciferase. X-Shining™ Luciferase is supplied as a lyophilisate (1% w/w X-Shining™ Luciferase) or as aqueous solution with glycerol (BX174908), and it may be stored for months at room temperature without significant loss of function. Find out more about our innovative X-Shining range here X-Shining | Innovation | Cymit Quimica Carbosynth.

Purity: (Spec. Activity. U/Mg) Min. 10^7

Color and Shape: Powder

Ref: 3D-BX180145