CAS 63368-54-7

5-(iodoacetamido)fluorescein

Description:

5-(Iodoacetamido)fluorescein is a fluorescent dye commonly used in biochemical applications, particularly in labeling and imaging studies. This compound features a fluorescein backbone, which is known for its strong fluorescence properties, making it suitable for various assays and microscopy techniques. The presence of the iodoacetamido group enhances its reactivity, allowing for covalent attachment to biomolecules such as proteins and peptides through nucleophilic substitution reactions. This characteristic is particularly useful in the development of fluorescent probes for tracking biological processes. The compound is typically soluble in organic solvents and exhibits a distinct absorption and emission spectrum, which can be exploited in fluorescence microscopy and flow cytometry. Additionally, its stability under physiological conditions makes it a valuable tool in cellular imaging and diagnostics. However, as with many fluorescent dyes, care must be taken regarding photobleaching and the potential for non-specific binding in complex biological systems. Overall, 5-(iodoacetamido)fluorescein is a versatile reagent in the field of biochemistry and molecular biology.

Formula: C₂₂H₁₄INO₆

InChI: InChI=1/C22H14INO6/c23-10-20(27)24-11-1-4-14(22(28)29)17(7-11)21-15-5-2-12(25)8-18(15)30-19-9-13(26)3-6-16(19)21/h1-9,25H,10H2,(H,24,27)(H,28,29)

SMILES: c1cc(c(cc1N=C(CI)O)c1c2ccc(cc2oc2cc(=O)ccc12)O)C(=O)O

Synonyms:

• 5-Iodoacetamidofluorescein (5-IAF)
• N-(3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-5-yl)-2-iodoacetamide
• 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-4-[(iodoacetyl)amino]benzoic acid

5-IAF

CAS: 63368-54-7

Formula: C₂₂H₁₄INO₆

Purity: 95%

Color and Shape: Solid

Molecular weight: 515.2541

Ref: IN-DA00E8VX

5-Iodoacetamido fluorescein

CAS: 63368-54-7

5-Iodoacetamido fluorescein

Color and Shape: Powder

Molecular weight: 515.25g/mol

Ref: 54-BII7022

5-IAF

CAS: 63368-54-7

5-IAF is a fluorescein derivative of indoleacetamide, a fluorescent probe that can be used to label proteins and others containing free thiols (cysteine side

Formula: C₂₂H₁₄INO₆

Purity: 98%

Color and Shape: Solid

Molecular weight: 515.25

Ref: TM-T14052

5-(Iodoacetamido)fluorescein

Controlled Product

CAS: 63368-54-7

Applications 5-(Iodoacetamido)fluorescein is a thiol reactive fluorescein derivative.

Formula: C₂₂H₁₄INO₆

Color and Shape: Neat

Molecular weight: 515.25

Ref: TR-I685518

5-(Iodoacetamido)fluorescein

CAS: 63368-54-7

5-(Iodoacetamido)fluorescein is a fluorescent probe that can be used as a substrate for the detection of proteolytic enzymes. It binds to the disulfide bond of serine protease and reacts with iodoacetic acid. This reaction leads to a biphasic response, with an initial fluorescence increase followed by a decrease in fluorescence intensity. The initial fluorescence increase is due to the formation of a stable 5-iodoacetylamino-2-aminopyridine intermediate, which is converted into 5-iodoacetamido-2-aminopyridine upon cleavage of the disulfide bond. Fluorescence emission from 5-(Iodoacetamido)fluorescein increases when it binds to tryptophan residues on actin filaments, which are found in large numbers in human liver cells. 5-(Iodoacetamido)fluorescein also binds to herpes simplex virus and

Formula: C₂₂H₁₄INO₆

Purity: Min. 95%

Color and Shape: Red Powder

Molecular weight: 515.25 g/mol

Ref: 3D-FI11400