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CAS 64285-73-0

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3,3′,5,5′-Tetramethylbenzidine dihydrochloride

Description:
3,3′,5,5′-Tetramethylbenzidine dihydrochloride (TMB) is an organic compound commonly used as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISA) and other biochemical applications. It is characterized by its ability to undergo oxidation, resulting in a color change that can be quantitatively measured, making it valuable for detecting peroxidase activity. TMB is a derivative of benzidine, featuring four methyl groups that enhance its solubility and stability in aqueous solutions. The dihydrochloride form indicates the presence of two hydrochloride ions, which can influence its solubility and reactivity. TMB is typically a pale yellow to colorless solid, and upon oxidation, it produces a blue-colored product, which is often used as an indicator in various assays. Safety considerations are important, as TMB and its derivatives can be potentially hazardous, necessitating appropriate handling and disposal measures. Overall, TMB is a crucial reagent in biochemical research and diagnostics due to its sensitivity and ease of use.
Formula:C16H20N2·2ClH
InChI:InChI=1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H
InChI key:InChIKey=NYNRGZULARUZCC-UHFFFAOYSA-N
SMILES:CC=1C=C(C=C(C)C1N)C2=CC(C)=C(N)C(C)=C2.Cl
Synonyms:
  • (TMB) Dihydrochloride
  • 3,3',5,5'-Tetramethyl-[1,1'-Biphenyl]-4,4'-DIAMINE, Dihydrochloride
  • 3,3',5,5'-Tetramethylbenzidine 2HCL
  • 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Monohydrate
  • 3,3',5,5'-Tetramethylbenzidine hydrochloride
  • 3,3',5,5'-Tetramethylbiphenyl-4,4'-diamine dihydrochloride
  • 3,3,5,5-Tetramethylbenzidine HCL
  • 3,3,5,5-Tetramethylbenzidine.2HCl
  • 3,3′,5,5′-Tetramethylbenzidine dihydrochloride
  • 4,4'-DIAMINO-3,3',5,5'-Tetramethylbiphenyl
  • See more synonyms
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