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CAS 65-61-2

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Acridine Orange

Description:
Acridine Orange is a synthetic fluorescent dye commonly used in biological and biochemical applications, particularly in microscopy and cell staining. It is characterized by its ability to intercalate into nucleic acids, allowing for the differentiation between live and dead cells based on their membrane integrity. The compound exhibits a strong fluorescence under ultraviolet light, with distinct emission spectra that vary depending on its binding state; it fluoresces green when bound to RNA and red when bound to DNA. Acridine Orange is soluble in water and organic solvents, making it versatile for various experimental conditions. Its chemical structure includes an acridine ring system, contributing to its planar configuration, which facilitates intercalation. Due to its properties, Acridine Orange is widely utilized in research for studying cellular processes, assessing cell viability, and detecting nucleic acids in various samples. However, caution is advised when handling this compound, as it can be mutagenic and potentially harmful to human health.
Formula:C17H20ClN3
InChI:InChI=1/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H
InChI key:InChIKey=VSTHNGLPHBTRMB-UHFFFAOYSA-N
SMILES:N(C)(C)C1=CC2=C(C=C3C(=N2)C=C(N(C)C)C=C3)C=C1.Cl
Synonyms:
  • 3,6-Acridinediamine, N,N,N',N'-tetramethyl-, monohydrochloride
  • 3,6-Acridinediamine, N3,N3,N6,N6-tetramethyl-, hydrochloride (1:1)
  • 3,6-Acridinediamine, N<sup>3</sup>,N<sup>3</sup>,N<sup>6</sup>,N<sup>6</sup>-tetramethyl-, hydrochloride (1:1)
  • 3,6-Bis(dimethylamino)acridine hydrochloride
  • Acridine Orange N
  • Acridine Orange NO
  • Acridine Orange NS
  • Acridine Orange R
  • Acridine orange
  • Acridine, 3,6-bis(dimethylamino)-, hydrochloride
  • See more synonyms
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