CAS 65022-15-3

Z-glycyl-L-proline-4-nitroanilide

Description:

Z-glycyl-L-proline-4-nitroanilide, with the CAS number 65022-15-3, is a synthetic peptide derivative commonly used in biochemical research, particularly in the study of proteolytic enzymes. This compound features a Z (benzyloxycarbonyl) protecting group on the amino group of glycine, which enhances its stability and solubility. The presence of L-proline contributes to its structural rigidity, while the 4-nitroanilide moiety serves as a chromogenic or fluorogenic reporter, making it useful in enzyme assays. The compound is typically characterized by its ability to undergo hydrolysis in the presence of specific proteases, releasing detectable products that can be quantified. Its molecular structure includes both peptide bonds and aromatic systems, which can influence its reactivity and interaction with biological macromolecules. Overall, Z-glycyl-L-proline-4-nitroanilide is valuable in the development of substrates for studying enzyme kinetics and mechanisms in various biological contexts.

Formula: C₂₁H₂₂N₄O₆

InChI: InChI=1/C21H22N4O6/c26-19(13-22-21(28)31-14-15-5-2-1-3-6-15)24-12-4-7-18(24)20(27)23-16-8-10-17(11-9-16)25(29)30/h1-3,5-6,8-11,18H,4,7,12-14H2,(H,22,28)(H,23,27)/t18-/m0/s1

SMILES: c1ccc(cc1)COC(=NCC(=O)N1CCC[C@H]1C(=O)Nc1ccc(cc1)N(=O)=O)O

Synonyms:

• Z-Gly-Pro-pNA
• Z-Gly-Pro-4-nitroanilide
• N-[(benzyloxy)carbonyl]glycyl-N-(4-nitrophenyl)-L-prolinamide

Z-Gly-Pro-pNA

CAS: 65022-15-3

Z-GP-pNA, chromogenic substrate for post-proline cleaving enzyme (prolyl endopeptidase).

Formula: C₂₁H₂₂N₄O₆

Purity: > 99%

Color and Shape: Light Yellow Powder

Molecular weight: 426.43

Ref: 01-4003649

Z-Gly-Pro-PNA

CAS: 65022-15-3

Formula: C₂₁H₂₂N₄O₆

Purity: 95%

Color and Shape: Solid

Molecular weight: 426.4226

Ref: IN-DA00H6B9

Benzyl (S)-(2-(2-((4-nitrophenyl)carbamoyl)pyrrolidin-1-yl)-2-oxoethyl)carbamate

CAS: 65022-15-3

Benzyl (S)-(2-(2-((4-nitrophenyl)carbamoyl)pyrrolidin-1-yl)-2-oxoethyl)carbamate

Purity: 95%

Molecular weight: 426.43g/mol

Ref: 54-OR1071642

Z-Glycyl-LProline-4-Nitroanilide (Z-Gly-Pro-4-Nitroanilide) extrapure, 98%

CAS: 65022-15-3

Formula: C₂₁H₂₂N₄O₆

Purity: min. 98.0%

Color and Shape: White to off-white, Powder

Molecular weight: 426.42

Ref: SR-15746

Z-Gly-Pro-pNA

CAS: 65022-15-3

Z-Gly-Pro-pNA is a synthetic substrate that has been used in the development of polyclonal antibodies against the surface protein of Stenotrophomonas maltophilia. The antibody, when immobilized to an insoluble support, was able to detect the bacterial cells within 10 hours. Z-Gly-Pro-pNA is a cyclic peptide with a proteolytic activity and an acidic pH optimum. It has been shown that Z-Gly-Pro-pNA can be hydrolysed by serine proteases such as trypsin and chymotrypsin.

Formula: C₂₁H₂₂N₄O₆

Purity: Min. 95%

Molecular weight: 426.42 g/mol

Ref: 3D-FG110719