CAS 65147-06-0

Boc-Ile-Glu-Gly-Arg-AMC

Description:

Boc-Ile-Glu-Gly-Arg-AMC is a synthetic peptide substrate commonly used in biochemical research, particularly in studies involving proteolytic enzymes. The name indicates that it is a derivative of the amino acids isoleucine (Ile), glutamic acid (Glu), glycine (Gly), and arginine (Arg), with a Boc (tert-butyloxycarbonyl) protecting group on the isoleucine residue. The AMC (7-amino-4-methylcoumarin) moiety serves as a fluorescent reporter, allowing for the monitoring of enzymatic activity through fluorescence assays. This compound is typically utilized in assays to study proteases, as the cleavage of the peptide bond by the enzyme releases the AMC group, resulting in an increase in fluorescence that can be quantitatively measured. The stability of the Boc group under certain conditions makes it suitable for various experimental setups. Overall, Boc-Ile-Glu-Gly-Arg-AMC is a valuable tool in the field of enzymology and peptide chemistry, aiding in the understanding of enzyme kinetics and substrate specificity.

Formula: C₃₄H₅₀N₈O₁₀

InChI: InChI=1/C34H50N8O10/c1-7-18(2)28(42-33(50)52-34(4,5)6)31(49)40-23(12-13-26(44)45)29(47)38-17-25(43)41-30(48)22(9-8-14-37-32(35)36)39-20-10-11-21-19(3)15-27(46)51-24(21)16-20/h10-11,15-16,18,22-23,28,39H,7-9,12-14,17H2,1-6H3,(H,38,47)(H,40,49)(H,42,50)(H,44,45)(H4,35,36,37)(H,41,43,48)/t18-,22-,23-,28-/m0/s1

SMILES: CC[C@H](C)[C@@H](C(=N[C@@H](CCC(=O)O)C(=NCC(=NC(=O)[C@H](CCCNC(=N)N)Nc1ccc2c(C)cc(=O)oc2c1)O)O)O)N=C(O)OC(C)(C)C

Synonyms:

• tertiary-Butyloxycarbonyl-isoleucyl-glutamyl-glycyl-arginyl-7-amino-4-methylcoumarin
• Biggam
• Boc-ile-glu-gly-arg-mca
• L-Argininamide, N-((1,1-dimethylethoxy)carbonyl)-L-isoleucyl-L-alpha-glutamylglycyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-
• N-(tert-butoxycarbonyl)-L-isoleucyl-L-alpha-glutamyl-N-{(2S)-5-[(diaminomethylidene)amino]-2-[(4-methyl-2-oxo-2H-chromen-7-yl)amino]pentanoyl}glycinamide
• Boc-Ile-Glu-Gly-Arg-Amc Acetate Salt

Boc-Ile-Glu-Gly-Arg-AMC

CAS: 65147-06-0

Boc-IEGR-AMC, a specific, highly fluorogenic substrate for clotting enzyme factor Xa (coagulation factor Xa). Boc-IEGR-AMC is also hydrolyzed by acrosin from the ascidian Halocynthia roretzi. The use of this substrate in the indirect determination of coagulation factor IX has been described.

Formula: C₃₄H₅₀N₈O₁₀

Purity: 97.8%

Color and Shape: White Powder

Molecular weight: 730.82

Ref: 01-4004961

Boc-Ile-Glu-Gly-Arg-AMC

CAS: 65147-06-0

Boc-IEGR-AMC: fluorogenic substrate for factor Xa and Halocynthia roretzi acrosin.

Formula: C₃₄H₅₀N₈O₁₀

Purity: 98%

Color and Shape: White Powder

Molecular weight: 730.81

Ref: TM-TP1129

Boc-Ile-Glu-Gly-Arg-AMC

CAS: 65147-06-0

Boc-Ile-Glu-Gly-Arg-AMC is a cell permeable inhibitor of protein interactions that binds to the peptide sequences of the extracellular domain of the epidermal growth factor receptor (EGFR). Boc-Ile-Glu-Gly-Arg-AMC can be used as a research tool to study protein interactions, as well as an inhibitor for EGFR. It is soluble in DMSO and water. This product has been shown to inhibit the activity of ion channels such as voltage dependent calcium channels, potassium channels, and sodium channels. Boc-Ile-Glu-Gly-Arg-AMC also inhibits antibody binding to its antigen.

Formula: C₃₄H₅₀N₈O₁₀

Purity: Min. 95%

Molecular weight: 730.81 g/mol

Ref: 3D-MIE-3094-V

Boc-Ile-Glu-Gly-Arg-AMC acetate salt

CAS: 65147-06-0

Boc-Ile-Glu-Gly-Arg-AMC acetate salt is a homologous peptide derived from soybean trypsin inhibitor. It has a molecular weight of 7,000 daltons and inhibits the activity of serine proteases, including kallikrein, plasmin, and thrombin. This peptide has been shown to be an inhibitor of heparin coagulation factor Xa and tissue factor in vitro. Boc-Ile-Glu-Gly-Arg-AMC acetate salt also has anticoagulant properties in vivo which are due to its ability to inhibit the synthesis of prothrombin.

Formula: C₃₄H₅₀N₈O₁₀·C₂H₄O₂

Purity: Min. 95%

Color and Shape: Solid

Molecular weight: 790.86 g/mol

Ref: 3D-FB110485