H-Gly-Arg-AMC, also known as Glycyl-Arginine-7-amido-4-methylcoumarin, is a synthetic peptide substrate commonly used in biochemical research, particularly in studies involving proteases. This compound features a glycine (Gly) and arginine (Arg) amino acid sequence linked to a 7-amido-4-methylcoumarin (AMC) fluorophore, which allows for fluorescence-based detection upon cleavage by specific enzymes. The presence of the AMC group makes it highly sensitive for monitoring enzymatic activity, as the release of the fluorophore results in an increase in fluorescence intensity. H-Gly-Arg-AMC is typically utilized in assays to study proteolytic activity, providing insights into enzyme kinetics and substrate specificity. Its stability and solubility in aqueous solutions make it suitable for various experimental conditions. Additionally, the compound's structure allows for the exploration of interactions between enzymes and substrates, contributing to the understanding of biological processes and potential therapeutic targets.

H-Gly-Arg-AMC

65147-19-5: H-Gly-Arg-AMC, also known as Glycyl-Arginine-7-amido-4-methylcoumarin, is a synthetic peptide substrate commonly used in biochemical research, particularly in studies involving proteases. This compound features a glycine (Gly) and arginine (Arg) amino acid sequence linked to a 7-amido-4-methylcoumarin (AMC) fluorophore, which allows for fluorescence-based detection upon cleavage by specific enzymes. The presence of the AMC group makes it highly sensitive for monitoring enzymatic activity, as the release of the fluorophore results in an increase in fluorescence intensity. H-Gly-Arg-AMC is typically utilized in assays to study proteolytic activity, providing insights into enzyme kinetics and substrate specificity. Its stability and solubility in aqueous solutions make it suitable for various experimental conditions. Additionally, the compound's structure allows for the exploration of interactions between enzymes and substrates, contributing to the understanding of biological processes and potential therapeutic targets.

Sensitive fluorogenic substrate for dipeptidyl aminopeptidase I (DPP I, cathepsin C).

<p>H-Gly-Arg-AMC hydrochloride salt is a substrate for the enzymes cathepsins B, H, and L. This compound has been used to measure protease activity in cell culture and as a diagnostic substrate for peptidases. The enzyme reaction can be monitored by measuring changes in the fluorescence of the product at 340 nm. The pH optimum for this enzyme is 7.4.</p>

CAS 65147-19-5

H-Gly-Arg-AMC

H-Gly-Arg-AMC

Ref: 01-4002196

H-Gly-Arg-AMC hydrochloride salt

Ref: 3D-FG110503