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CAS 6578-06-9

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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

Description:
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, with the CAS number 6578-06-9, is a chemical compound commonly used as a substrate in biochemical assays, particularly in the detection of alkaline phosphatase activity. This compound is characterized by its indole structure, which is modified by the presence of bromine and chlorine substituents, enhancing its reactivity. The phosphate group in its structure makes it a phosphate ester, which is crucial for its function as a substrate. Upon enzymatic hydrolysis by alkaline phosphatase, it produces a colored product, facilitating visual detection and quantification. The p-toluidine salt form indicates that it is paired with p-toluidine, which can influence solubility and stability in various solvents. This compound is typically utilized in molecular biology and biochemistry laboratories for enzyme assays and is valued for its specificity and sensitivity in detecting enzymatic activity. Proper handling and storage conditions are essential to maintain its integrity and effectiveness in experimental applications.
Formula:C8H6BrClNO4P·C7H9N
InChI:InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key:InChIKey=QEIFSLUFHRCVQL-UHFFFAOYSA-N
SMILES:O(P(=O)(O)O)C=1C=2C(NC1)=CC=C(Br)C2Cl.CC1=CC=C(N)C=C1
Synonyms:
  • 1H-Indol-3-ol, 5-bromo-4-chloro-, 3-(dihydrogen phosphate), compd. with 4-methylbenzenamine (1:1)
  • 1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1)
  • 5-Bromo-4-Bhloro-3-Indolylphosphate4-toluidinesalt
  • 5-Bromo-4-Chloro-1H-Indol-3-Yl Dihydrogen Phosphate - 4-Methylaniline (1:1)
  • 5-Bromo-4-Chloro-1H-Indol-3-Yl Phosphate P-Toluidine
  • 5-Bromo-4-Chloro-3'-Indolyphosphate P-Toluidine Salt
  • 5-Bromo-4-Chloro-3-Indolyl Hydrogen Phosphate--P-Toluidine (1:1)
  • 5-Bromo-4-Chloro-3-Indolyl Phosphate P-Toluidine
  • 5-Bromo-4-Chloro-3-Indolyl Phosphate, Compounded With 4-Methylbenzenamine
  • 5-Bromo-4-Chloro-3-Indolyl-Phosphate 4-Toluidine Salt
  • See more synonyms
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Purity (%)
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100
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0
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50
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90
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95
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100
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