CAS 69-78-3

5,5′-Dithiobis[2-nitrobenzoic acid]

Description:

5,5′-Dithiobis[2-nitrobenzoic acid], commonly referred to as DTNB, is a chemical compound widely used in biochemistry and molecular biology. It is characterized by its yellow crystalline appearance and is soluble in water and organic solvents. DTNB serves as a reagent for the detection of thiol groups in proteins and peptides due to its ability to form a colored product upon reaction with free sulfhydryl groups. The compound features two 2-nitrobenzoic acid moieties linked by a disulfide bond, which is responsible for its reactivity. DTNB is often employed in assays to quantify protein concentration and to study enzyme kinetics involving thiol-containing substrates. Additionally, it has applications in the field of drug development and biochemical research. Safety considerations include handling it with care, as it can be harmful if ingested or inhaled, and appropriate personal protective equipment should be used. Overall, DTNB is a valuable tool in biochemical analysis and research.

Formula: C₁₄H₈N₂O₈S₂

InChI: InChI=1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChI key: InChIKey=KIUMMUBSPKGMOY-UHFFFAOYSA-N

SMILES: C(O)(=O)C1=C(N(=O)=O)C=CC(SSC2=CC(C(O)=O)=C(N(=O)=O)C=C2)=C1

Synonyms:

• 2,2'-Dinitro-5,5'-dithiodibenzoic acid
• 3,3'-Disulfanediylbis(6-Nitrobenzoate)
• 3,3'-Disulfanediylbis(6-Nitrobenzoic Acid)
• 3,3'-Dithiobis(6-nitrobenzoic acid)
• 3,3'-Dithiobis[6-nitrobenzoe]saure
• 3-Carboxy-4-nitrophenyl disulfide
• 3-Carboxy-4-nitrophenyl disulphide
• 5,5-Dithio-Bis(2-Nitrobenzoic Acid)
• Acide 3,3'-dithiobis[6-nitrobenzoique]
• Acido 3,3'-Ditiobis[6-Nitrobenzoico]
• Ba 2767
• Benzoic acid, 3,3'-dithiobis[6-nitro-
• Bis(3-Carboxy-4-Nitrophenyl) Disulfide
• Bis(3-carboxy-4-nitrophenyl) disulphide
• Dtnb
• Ellman'S Reagent
• Ellmann'S Reagent
• Ellmanns
• Ellmans
• Ellmans'reagent
• Reagents, Ellman's
• 5,5'-DITHIOBIS(2-NITROBENZOIC ACID)

5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups]

CAS: 69-78-3

Formula: C₁₄H₈N₂O₈S₂

Purity: >98.0%(T)(HPLC)

Color and Shape: Light yellow to Yellow powder to crystal

Molecular weight: 396.34

Ref: 3B-D0944

5,5'-Dithiobis(2-nitrobenzoic acid), 99%

CAS: 69-78-3

Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reage

Formula: C₁₄H₈N₂O₈S₂

Purity: 99%

Color and Shape: Pale yellow to pale cream, Powder

Molecular weight: 396.36

Ref: 02-A14331

DTNB

CAS: 69-78-3

DTNB, or Ellman's Reagent, quantifies thiol groups and measures low-mass thiols like glutathione in pure and biological samples.

Formula: C₁₄H₈N₂O₈S₂

Purity: 97.11% - 99.51%

Color and Shape: Yellow Solid

Molecular weight: 396.35

Ref: TM-T15177

Benzoic acid, 3,​3'-​dithiobis[6-​nitro-

CAS: 69-78-3

Formula: C₁₄H₈N₂O₈S₂

Purity: 95%

Color and Shape: Solid

Molecular weight: 396.3519

Ref: IN-DA00IBW2

DTNB

CAS: 69-78-3

DTNB

Purity: ≥95%

Molecular weight: 396.35g/mol

Ref: 54-BUP19334

5,5''-Dithio-bis(2-nitrobenzoic acid)

CAS: 69-78-3

Formula: C₁₄H₈N₂O₈S₂

Purity: (HPLC) ≥ 98.5%

Color and Shape: Off-white to yellow powder or crystals

Molecular weight: 396.36

Ref: 7W-GK9458

5,5'-Dithiobis(2-nitrobenzoic acid)

CAS: 69-78-3

5,5'-Dithiobis(2-nitrobenzoic acid)

Formula: C₁₄H₈N₂O₈S₂

Purity: 98%

Color and Shape: yellow solid

Molecular weight: 396.35191g/mol

Ref: 54-BID2180

5,5'-Dithiobis(2-nitrobenzoic acid)

CAS: 69-78-3

DTNB is known as Ellman's reagent. It is used for the colorimetric determination of thiol groups in biological samples.It is fairly soluble in water. Colorless DTNB is converted to yellow 5-Mercapto-2-nitrobenzoic acid in the presence of thiol compounds. For example, DTNB in combination with S-acetylthiocholine iodide as the substrate is widely used to assay choline esterase activity.

Formula: C₁₄H₈N₂O₈S₂

Purity: Min. 98.0 Area-%

Color and Shape: Yellow Powder

Molecular weight: 396.35 g/mol

Ref: 3D-FD11353

DTNB

CAS: 69-78-3

Formula: C₁₄H₈N₂O₈S₂

Purity: 95%

Color and Shape: Solid, Light yellow powder

Molecular weight: 396.34

Ref: 10-F047885

5,5’-Dithiobis(2-Nitro Benzoic Acid) extrapure (DTNB), 98%

CAS: 69-78-3

Formula: C₁₄H₈N₂O₈S₂

Purity: min. 98 %

Color and Shape: Yellow, Crystalline powder, Clear, Yellow

Molecular weight: 396.35

Ref: SR-32363

3,3'-Dithiobis[6-nitrobenzoic Acid]

CAS: 69-78-3

Applications 3,3'-Dithiobis[6-nitrobenzoic Acid is an membrane-impermeant thiol/disulfide exchange blocker. 3,3'-Dithiobis[6-nitrobenzoic Acid is also used to quantify the number or concentration of thiol groups in a sample.
References Calderon, M.N., et al.: Intervirol., 55, 451 (2012); Li, J.M., et al.: J. Material. Chem., 22, 12100 (2012);

Formula: C₁₄H₈N₂O₈S₂

Color and Shape: Neat

Molecular weight: 396.35

Ref: TR-D479840