Ac-Ala-Ala-Pro-Phe-pNA, also known as N-acetyl-Ala-Ala-Pro-Phe-p-nitroanilide, is a synthetic peptide that serves as a substrate in biochemical assays, particularly for studying proteolytic enzymes. This compound consists of a sequence of amino acids, specifically alanine (Ala), proline (Pro), and phenylalanine (Phe), with an acetyl group at the N-terminus and a p-nitroanilide (pNA) moiety at the C-terminus. The acetylation enhances its stability and solubility, while the pNA group is a chromogenic marker that allows for the quantification of enzymatic activity through colorimetric assays. The peptide's structure contributes to its specificity and reactivity with various proteases, making it valuable in research and diagnostic applications. Additionally, the presence of the aromatic pNA group provides a distinct absorbance peak, facilitating the monitoring of reactions in real-time. Overall, Ac-Ala-Ala-Pro-Phe-pNA is an important tool in enzymology and peptide chemistry, aiding in the understanding of enzyme kinetics and substrate specificity.

Ac-Ala-Ala-Pro-Phe-pNA

70967-94-1: Ac-Ala-Ala-Pro-Phe-pNA, also known as N-acetyl-Ala-Ala-Pro-Phe-p-nitroanilide, is a synthetic peptide that serves as a substrate in biochemical assays, particularly for studying proteolytic enzymes. This compound consists of a sequence of amino acids, specifically alanine (Ala), proline (Pro), and phenylalanine (Phe), with an acetyl group at the N-terminus and a p-nitroanilide (pNA) moiety at the C-terminus. The acetylation enhances its stability and solubility, while the pNA group is a chromogenic marker that allows for the quantification of enzymatic activity through colorimetric assays. The peptide's structure contributes to its specificity and reactivity with various proteases, making it valuable in research and diagnostic applications. Additionally, the presence of the aromatic pNA group provides a distinct absorbance peak, facilitating the monitoring of reactions in real-time. Overall, Ac-Ala-Ala-Pro-Phe-pNA is an important tool in enzymology and peptide chemistry, aiding in the understanding of enzyme kinetics and substrate specificity.

<p>Ac-Ala-Ala-Pro-Phe-pNA is a serine protease inhibitor that has been shown to inhibit chymotrypsin, elastase, and other serine proteases. It is benzoylated to protect it from proteolytic degradation in the gastrointestinal tract and can be used as a therapeutic treatment for inflammatory bowel disease, pancreatitis, or other chronic diseases. Ac-Ala-Ala-Pro-Phe-pNA can be inactivated by thermal treatment (e.g., boiling) or by chemical reduction (e.g., sodium borohydride).</p>

CAS 70967-94-1

Ac-Ala-Ala-Pro-Phe-pNA

Ac-Ala-Ala-Pro-Phe-pNA

Ref: 3D-FA110692