Glutaryl-Gly-Gly-Phe-AMC, with the CAS number 70996-06-4, is a synthetic peptide substrate commonly used in biochemical research, particularly in the study of proteolytic enzymes. This compound consists of a glutaryl group linked to a sequence of amino acids: glycine (Gly), phenylalanine (Phe), and an amide moiety (AMC) that serves as a fluorogenic reporter. The presence of the glutaryl group enhances the peptide's stability and solubility, while the AMC group allows for the detection of enzymatic activity through fluorescence upon cleavage by specific proteases. This substrate is particularly valuable in assays that measure the activity of enzymes such as caspases or other proteases, providing insights into various biological processes, including apoptosis and protein turnover. Its characteristics include a relatively low molecular weight, high specificity for certain enzymes, and the ability to produce a measurable fluorescent signal, making it a useful tool in both basic and applied research in biochemistry and molecular biology.

Glutaryl-Gly-Gly-Phe-AMC

70996-06-4: Glutaryl-Gly-Gly-Phe-AMC, with the CAS number 70996-06-4, is a synthetic peptide substrate commonly used in biochemical research, particularly in the study of proteolytic enzymes. This compound consists of a glutaryl group linked to a sequence of amino acids: glycine (Gly), phenylalanine (Phe), and an amide moiety (AMC) that serves as a fluorogenic reporter. The presence of the glutaryl group enhances the peptide's stability and solubility, while the AMC group allows for the detection of enzymatic activity through fluorescence upon cleavage by specific proteases. This substrate is particularly valuable in assays that measure the activity of enzymes such as caspases or other proteases, providing insights into various biological processes, including apoptosis and protein turnover. Its characteristics include a relatively low molecular weight, high specificity for certain enzymes, and the ability to produce a measurable fluorescent signal, making it a useful tool in both basic and applied research in biochemistry and molecular biology.

<p>Glutaryl-Gly-Gly-Phe-AMC is a proteolytic peptide which is used in the study of the proteasome. It has been shown to have affinity for the proteasome, and can be used as a monoclonal antibody for both immunohistochemical analysis and two-dimensional electrophoresis. The peptide was sequenced and found to have no homology to any known protein or peptide. Glutaryl-Gly-Gly-Phe-AMC has been shown to bind specifically to β amyloid (Aβ) subunits in cell homogenates and cytosol, as well as nuclei in tissue sections from AD brain. In addition, it has been shown that this peptide can be fluorescently labeled with Alexa Fluor 488 or 594 dye for use in microscopy studies.</p>

CAS 70996-06-4

Glutaryl-Gly-Gly-Phe-AMC

Glutaryl-Gly-Gly-Phe-AMC

Ref: 3D-FG110501