CAS 71418-45-6

Bromobimane q

Description:

Bromobimane, with the CAS number 71418-45-6, is a fluorescent compound commonly used in biochemical applications, particularly for labeling thiols and other nucleophiles in biological systems. It features a bimane structure, which is a bicyclic compound that exhibits strong fluorescence properties, making it valuable for various analytical techniques, including fluorescence microscopy and flow cytometry. The presence of the bromine atom enhances its reactivity, allowing it to form stable conjugates with thiol groups, which are abundant in proteins and other biomolecules. This reactivity is crucial for studying protein interactions, cellular processes, and redox states in living organisms. Additionally, Bromobimane is characterized by its relatively low toxicity, making it suitable for use in biological research. Its fluorescence can be excited by specific wavelengths, enabling sensitive detection and quantification of labeled targets. Overall, Bromobimane serves as a powerful tool in the field of biochemistry and molecular biology for probing cellular functions and dynamics.

Formula: C₁₃H₁₉BrN₃O₂·Br

InChI: InChI=1S/C13H19BrN3O2.BrH/c1-8-10(6-14)15-11(7-17(3,4)5)9(2)13(19)16(15)12(8)18;/h6-7H2,1-5H3;1H/q+1;/p-1

InChI key: InChIKey=FCRPMMVPBVOKQS-UHFFFAOYSA-M

SMILES: C([N+](C)(C)C)C=1N2N(C(=O)C1C)C(=O)C(C)=C2CBr.[Br-]

Synonyms:

• Bromobimane q
• 1H,7H-Pyrazolo[1,2-a]pyrazole-3-methanaminium, 5-(bromomethyl)-N,N,N,2,6-pentamethyl-1,7-dioxo-, bromide (1:1)
• Thiolyte MQ
• 1H,7H-Pyrazolo[1,2-a]pyrazole-3-methanaminium, 5-(bromomethyl)-N,N,N,2,6-pentamethyl-1,7-dioxo-, bromide

QBBR

CAS: 71418-45-6

Formula: C₁₃H₁₉Br₂N₃O₂

Color and Shape: Solid

Molecular weight: 409.1169

Ref: IN-DA00FB51

Bromotrimethylammoniumbimane bromide

CAS: 71418-45-6

Molecular weight: 409.12

Ref: 7W-GT2928

Bromotrimethylammoniumbimame bromide

CAS: 71418-45-6

Bromotrimethylammoniumbimame bromide

Formula: C₁₃H₁₉BrN₃O₂·Br

Purity: ≥95%

Color and Shape: light yellow powder

Molecular weight: 409.12g/mol

Ref: 54-OR8851T

Bromotrimethylammoniumbimane Bromide

Controlled Product

CAS: 71418-45-6

Stability Moisture, Light Sensitive - Desiccate and protect from light
Applications A charged fluoresent sulfhydryl active reagent. Bromotrimethylammoniumbimane is a derivative of monobromobimane containing a positive charge which permits separation of its conjugates by electrophoresis or cation-exchange chromatography. The membrane permeability of this charged bimane as well as its ability to access sites within a protein may differ from that of the uncharged bimane probes. These reagents are also useful for detecting the distribution of protein thiols in cells before and after chemical reduction with disulfides.Fluorescence: max. Abs. l = 398nm, max. Em. l = 475-485 (when reacted with thiol); e x 10-3: 5.0
References Meth. Enzymol., 143, 76 (1987), Proc. Natl. Acad. Sci. USA, 82, 1658 (1985), J. Leukocyte Biol., 45, 177 (1989), Arch. Biochem. Biophys., 282, 309 (1990), Zhu, Q-S., et al.: J.B.C., 278, 3112 (2003)

Formula: C₁₃H₁₉BrN₃O₂·Br

Color and Shape: Yellow

Molecular weight: 409.12

Ref: TR-B688500

Bromotrimethylammoniumbimane bromide

CAS: 71418-45-6

Bromotrimethylammoniumbimane bromide is a fluorescent molecule that can be used as a probe for protease activity. It is also used to study the α1 subunit of the human histone deacetylase (HDAC) complex, which plays an important role in HIV infection and autoimmune diseases. The redox potential of Bromotrimethylammoniumbimane bromide has been shown to be dependent on its basic structure, with the disulfide bond being the most reactive. This chemical has been shown to have cytosolic Ca2+-dependent effects on cell signaling pathways, including induction of apoptosis and modulation of protein phosphatases.

Formula: C₁₃H₁₉Br₂N₃O₂

Purity: Min. 95%

Color and Shape: Powder

Molecular weight: 409.12 g/mol

Ref: 3D-FB19336