CAS 71973-79-0

N-succinyl-ala-ala-phe 7-amido-4-*methylcoumarin

Description:

N-succinyl-ala-ala-phe 7-amido-4-methylcoumarin is a synthetic peptide substrate commonly used in biochemical assays, particularly for studying proteolytic enzymes. This compound features a succinyl group that enhances its solubility and stability in aqueous solutions, making it suitable for various experimental conditions. The presence of the amino acid sequence alanine-alanine-phenylalanine (ala-ala-phe) provides specificity for certain proteases, allowing researchers to monitor enzyme activity through fluorescence. The 7-amido-4-methylcoumarin moiety serves as a fluorogenic reporter, emitting fluorescence upon cleavage by the target enzyme, which is useful for real-time monitoring in assays. This compound is typically utilized in research settings to investigate enzyme kinetics, substrate specificity, and the effects of inhibitors. Its stability, solubility, and fluorescent properties make it a valuable tool in molecular biology and biochemistry. Proper handling and storage conditions are essential to maintain its integrity and reactivity in experimental applications.

Formula: C₂₉H₃₂N₄O₈

InChI: InChI=1/C29H32N4O8/c1-16-13-26(37)41-23-15-20(9-10-21(16)23)32-29(40)22(14-19-7-5-4-6-8-19)33-28(39)18(3)31-27(38)17(2)30-24(34)11-12-25(35)36/h4-10,13,15,17-18,22H,11-12,14H2,1-3H3,(H,30,34)(H,31,38)(H,32,40)(H,33,39)(H,35,36)

SMILES: Cc1cc(=O)oc2cc(ccc12)N=C(C(Cc1ccccc1)N=C(C(C)N=C(C(C)N=C(CCC(=O)O)O)O)O)O

Synonyms:

• Suc-Ala-Ala-Phe-AMC
• N-(3-carboxypropanoyl)alanylalanyl-N-(4-methyl-2-oxo-2H-chromen-7-yl)phenylalaninamide

Suc-Ala-Ala-Phe-AMC

CAS: 71973-79-0

Suc-AAF-AMC, a highly sensitive fluorogenic chymotrypsin substrate. It is also hydrolyzed by neutral metalloendopeptidases, such as thermolysin and enkephalinase (neprilysin, NEP 24.11). Suc-AAF-MCA has also been used for the chymotrypsin-like activity of the 20S proteasome.

Formula: C₂₉H₃₂N₄O₈

Purity: > 98%

Color and Shape: White Powder

Molecular weight: 564.6

Ref: 01-4006306

Suc-Ala-Ala-Phe-AMC

CAS: 71973-79-0

Suc-Ala-Ala-Phe-AMC, a fluorogenic chymotrypsin substrate hydrolyzable by endopeptidase, has applications in vivo for assaying the acrosome reaction and in

Formula: C₂₉H₃₂N₄O₈

Color and Shape: Solid

Molecular weight: 564.59

Ref: TM-T78407

Suc-Ala-Ala-Phe-AMC

CAS: 71973-79-0

Suc-Ala-Ala-Phe-AMC is a synthetic substrate that has been used to measure angiotensinase activity in blood pressure regulation. It is hydrolysed by the enzyme serine protease and the rate of hydrolysis is proportional to the concentration of angiotensin. Suc-Ala-Ala-Phe-AMC can be used as a substitute for angiotensin I, which is not readily available for experimental use. Suc-Ala-Ala-Phe-AMC does not contain any amino acids that are essential for life, and so it can be used in experiments with animals without causing them any harm.

Formula: C₂₉H₃₂N₄O₈

Purity: Min. 95%

Color and Shape: Powder

Molecular weight: 564.59 g/mol

Ref: 3D-FS110519