CAS 72088-94-9

Carboxyfluorescein

Description:

Carboxyfluorescein is a fluorescent dye commonly used in biological and chemical applications, particularly in fluorescence microscopy and flow cytometry. It is characterized by its bright green fluorescence, which is due to its conjugated structure that allows for efficient light absorption and emission. The substance is soluble in water and exhibits pH-dependent fluorescence, making it useful for studying cellular processes and membrane dynamics. Carboxyfluorescein can be used as a tracer in various assays, including those that measure cell viability, proliferation, and membrane integrity. Its ability to form stable conjugates with proteins and other biomolecules enhances its utility in labeling and tracking experiments. Additionally, it is often employed in the synthesis of fluorescent probes and in the development of biosensors. Safety considerations include handling it with care, as with any chemical dye, to avoid potential skin or eye irritation. Overall, carboxyfluorescein is a versatile tool in both research and diagnostic applications due to its distinct optical properties and compatibility with biological systems.

Formula: C₂₁H₁₂O₇

InChI: InChI=1/2C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21;22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h2*1-9,22-23H,(H,24,25)

SMILES: c1cc2c(cc1C(=O)O)C(=O)OC12c2ccc(cc2Oc2cc(ccc12)O)O.c1cc2c(cc1C(=O)O)C1(c3ccc(cc3Oc3cc(ccc13)O)O)OC2=O

Synonyms:

• 3',6'-Dihydroxy-1-Oxo-Spiro[Isobenzofuran-3,9'-Xanthene]-5-Carboxylic Acid
• 3',6'-Dihydroxy-3-Oxo-Spiro[Isobenzofuran-1,9'-Xanthene]-5-Carboxylic Acid
• 3',6'-dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one-acetic acid (1:1)
• 3′,6′-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-ar-carboxylic acid
• 4(5)-Carboxyfluorescein
• 5(6)-Carboxyfluorescein
• 5(6)-Carboxyfluorescein, For Fluorescenc E
• 5(6)-Fam
• 5(6)Carboxy fluorescein
• 5-(And-6)-Carboxyfluorescein
• 9'-[9H]Xanthene]-Ar-Carboxylicacid,3',6'-Dihydroxy-Spiro[Isobenzofuran-1(3H
• C 194
• Carboxyfluorescein
• Fluoresceincarboxylic acid
• Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo-

5(6)-Carboxyfluorescein, mixture of isomers, 97%

CAS: 72088-94-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Formula: C₂₁H₁₂O₇

Purity: 97%

Color and Shape: Orange to brown or orange-red or orange-brown, Powder or crystalline powder and/or chunks

Molecular weight: 376.32

Ref: 02-L13439

5(6)-Carboxyfluorescein, mixture of isomers

CAS: 72088-94-9

Formula: C₄₂H₂₄O₁₄

Purity: 95%

Color and Shape: Solid

Molecular weight: 752.6316

Ref: IN-DA00IKJ4

5(6)-Carboxyfluorescein

CAS: 72088-94-9

5(6)-Carboxyfluorescein

Formula: ·C₂₁H₁₂O₇·C₂₁H₁₂O₇

Purity: 98% (nmr) (Typical Value in Batch COA)

Color and Shape: pale red solid

Molecular weight: 752.63g/mol

Ref: 54-BIC1073

5(6)-Carboxyfluorescein

CAS: 72088-94-9

5(6)-Carboxyfluorescein

Purity: ≥95%

Molecular weight: 376.32g/mol

Ref: 54-BUP18955

5(6)-Carboxyfluorescein

CAS: 72088-94-9

5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) contains a carboxylic acid that can be used to react with primary amines via carbodiimide

Formula: C₂₁H₁₂O₇

Purity: 97.57% - 98.85%

Color and Shape: Off-White Solid

Molecular weight: 376.32

Ref: TM-T18893

5(6)-Carboxyfluorescein

CAS: 72088-94-9

Formula: C₂₁H₁₂O₇

Purity: ≥ 95%

Color and Shape: Yellow to dark orange powder

Molecular weight: 376.32

Ref: 7W-GE4671

5(6)-Carboxyfluorescein

Controlled Product

CAS: 72088-94-9

Applications 5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent.
References Theisen, P., et al.: Tetrahedron Lett., 33, 5033 (1992), Mattingly, P.: Bioconjgate Chem., 3, 430 (1992, Bertrand, R., et al.: Biochemistry, 34, 9500 (1995)

Formula: C₂₁H₁₂O₇

Color and Shape: Dark Brown

Molecular weight: 752.63

Ref: TR-C178315

5(6)-Carboxyfluorescein - mixture

CAS: 72088-94-9

6-Carboxyfluorescein is a fluorescent dye widely used in PCR methodologies, it is used to label the 5' end of oligonucleotides as a reporter in the presence of a quencher at the 3' end (dual labelled probe). During the amplification process, the dye is cleaved, and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. It is used to tag DNA and RNA for in vivo and in vitro applications, as well as for structural studies and RNA-protein interactions.

Formula: C₂₁H₁₂O₇

Purity: Min. 95%

Color and Shape: Powder

Molecular weight: 376.32 g/mol

Ref: 3D-FC19772

5(6)-Carboxyfluorescein

CAS: 72088-94-9

5(6)-Carboxyfluorescein is a fluorescent dye that can be used as a building block for the synthesis of more complex compounds. It is also used as a reagent for research and detection in various chemical reactions. 5(6)-Carboxyfluorescein has been shown to react with certain amines, alcohols, and thiols to form useful scaffolds for drug discovery. This compound is also used as an intermediate in the production of pharmaceutical drugs and agrochemicals.

Formula: C₂₁H₁₂O₇

Molecular weight: 376.33 g/mol

Ref: 3D-C-1825

5(6)-Carboxyfluorescein

CAS: 72088-94-9

M03602 - 5(6)-Carboxyfluorescein

Formula: C₄₂H₂₄O₁₄

Purity: 95%

Color and Shape: Solid, Orange solid

Molecular weight: 752.64

Ref: 10-M03602