CAS 7300-59-6

γ-Glutamyl-p-nitroanilide

Description:

γ-Glutamyl-p-nitroanilide is a synthetic compound commonly used in biochemical assays, particularly for the detection of enzymes such as γ-glutamyltransferase. It is characterized by its structure, which includes a γ-glutamyl group linked to a p-nitroaniline moiety. This compound is typically a yellow crystalline solid, reflecting its p-nitroaniline component, which contributes to its chromogenic properties. In aqueous solutions, it can undergo hydrolysis, releasing p-nitroaniline, which can be quantitatively measured due to its distinct color change. The compound is soluble in organic solvents and has limited solubility in water, making it suitable for various laboratory applications. Its use in enzyme assays is based on the principle that the enzymatic cleavage of the γ-glutamyl bond results in a measurable increase in absorbance, allowing for the quantification of enzyme activity. Safety precautions should be taken when handling this compound, as p-nitroaniline is known to be toxic and potentially carcinogenic.

Formula: C₁₁H₁₃N₃O₅

InChI: InChI=1S/C11H13N3O5/c12-9(11(16)17)5-6-10(15)13-7-1-3-8(4-2-7)14(18)19/h1-4,9H,5-6,12H2,(H,13,15)(H,16,17)/t9-/m0/s1

InChI key: InChIKey=WMZTYIRRBCGARG-VIFPVBQESA-N

SMILES: N(=O)(=O)C1=CC=C(NC(CC[C@@H](C(O)=O)N)=O)C=C1

Synonyms:

• (2S)-2-Azaniumyl-5-(4-nitroanilino)-5-oxopentanoate
• (S)-2-Amino-5-((4-nitrophenyl)amino)-5-oxopentanoic acid
• (S)-2-Amino-5-((4-nitrophenyl)amino)-5-oxopentanoicacid
• <span class="text-smallcaps">L</span>-Glutamic acid γ-p-nitroanilide
• <span class="text-smallcaps">L</span>-Glutamine, N-(4-nitrophenyl)-
• <span class="text-smallcaps">L</span>-Glutamyl-4-nitroanilide
• <span class="text-smallcaps">L</span>-γ-Glutamyl-p-nitroanilide
• Ccris 8420
• Gamma-L-Glutamyl-4-Nitroanilide
• Glutaranilic acid, 2-amino-4′-nitro-, <span class="text-smallcaps">L</span>-
• L-2-Amino-4'-nitroglutaranilic acid
• L-Glutamine, N-(4-nitrophenyl)-
• L-gamma-Glutamyl-p-nitroanilide
• N-(4-Nitrophenyl)-<span class="text-smallcaps">L</span>-glutamine
• N-(4-Nitrophenyl)-L-glutamine
• gamma-Glutamine-4-nitroanilide
• γ-<span class="text-smallcaps">L</span>-Glu-p-nitroanilide
• γ-<span class="text-smallcaps">L</span>-Glutamyl-p-nitroanilide
• γ-Glutamyl-p-nitroanilide
• Glutaranilic acid, 2-amino-4′-nitro-, L-

L-γ-Glutamyl-p-nitroanilide Monohydrate

CAS: 7300-59-6

Formula: C₁₁H₁₃N₃O₅·H₂O

Purity: >98.0%(T)(HPLC)

Color and Shape: White to Light yellow powder to crystal

Molecular weight: 285.26

Ref: 3B-G0065

(S)-2-Amino-5-((4-nitrophenyl)amino)-5-oxopentanoic acid

CAS: 7300-59-6

Formula: C₁₁H₁₃N₃O₅

Purity: 98%

Color and Shape: Solid

Molecular weight: 267.2380

Ref: IN-DA003QQI

γ-L-Glutamyl-4-Nitroanilide

CAS: 7300-59-6

γ-L-Glutamyl-4-Nitroanilide

Purity: 98%

Molecular weight: 267.24g/mol

Ref: 54-OR1013416

H-Glu(pNA)-OH

CAS: 7300-59-6

Gamma-GPNA is a highly specific and sensitive substrate commonly employed for assessing gamma-glutamyl transferase activity in both serum and tissues, particularly through automated colorimetric methods. In this context, the dipeptide Gly-Gly serves as the glutamyl acceptor, facilitating the enzymatic reaction and subsequent detection of gamma-glutamyl transferase activity. This substrate has been widely utilized in enzymatic assays due to its specificity and sensitivity, allowing for accurate determination of gamma-glutamyl transferase levels in various biological samples.

Formula: C₁₁H₁₃N₃O₅

Purity: Min. 95%

Color and Shape: Powder

Molecular weight: 267.24 g/mol

Ref: 3D-FG110726

L-Glutamic Acid γ-p-Nitroanilide-d4

Controlled Product

CAS: 7300-59-6

Formula: C₁₁D₄H₉N₃O₅

Color and Shape: Neat

Molecular weight: 271.263

Ref: TR-G597052

L-Glutamic Acid γ-p-Nitroanilide

Controlled Product

CAS: 7300-59-6

Applications L-Glutamic Acid γ-p-Nitroanilide is a substrate for γ-glutamyltransferase (GGT), which is an important enzyme with wide applications in biocatalysis and clinical diagnosis.
References Li, Zh., et al.: J. Zh. Univer. Sci., 12, 180 (2011); Qin, D., et al.: Huagong Xuebao , 62, 378 (2011);

Formula: C₁₁H₁₃N₃O₅

Color and Shape: Neat

Molecular weight: 267.24

Ref: TR-G597050