CAS 7365-45-9

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

Description:

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, commonly known as HEPES, is a zwitterionic buffering agent widely used in biological and biochemical research. It has a pKa value around 7.5, making it particularly effective for maintaining physiological pH in cell culture and biochemical assays. HEPES is soluble in water and exhibits good stability under physiological conditions, which is crucial for experiments requiring consistent pH levels. Its structure features a piperazine ring, a sulfonic acid group, and a hydroxyethyl side chain, contributing to its buffering capacity. HEPES is non-toxic to cells, making it suitable for various applications, including protein purification and enzyme assays. Additionally, it is less prone to interfere with biological processes compared to other buffers, such as phosphate buffers. However, it is important to note that HEPES can absorb UV light, which may affect certain spectroscopic analyses. Overall, HEPES is a versatile and widely utilized buffer in laboratory settings, particularly in the fields of molecular biology and biochemistry.

Formula: C₈H₁₈N₂O₄S

InChI: InChI=1/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)/p+1

InChI key: InChIKey=JKMHFZQWWAIEOD-UHFFFAOYSA-N

SMILES: C(CS(=O)(=O)O)N1CCN(CCO)CC1

Synonyms:

• 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
• 1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonic acid
• 2-[4-(2-Hydroxyethyl)-1-Piperazinyl]Ethanesulfonic Acid
• 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid
• 2-[4-(2-Hydroxyethyl)Piperazin-4-Ium-1-Yl]Ethanesulfonate
• 2-[4-(2-Hydroxyethyl)Piperazinediium-1-Yl]Ethanesulfonate
• 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
• 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
• 2-[4-(2-Hydroxyethyl)piperazinyl]ethanesulfonic acid
• 2-[4-(Hydroxyethyl)-1-piperazinyl]ethane sulfonic acid
• 4-(2-Hydroxyethy1)-1-piperazineethanesulfonic acid
• 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic Acid
• 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonicacid
• 4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid
• 4-(2-Hydroxyethyl)Piperazin-1-Ylethanesulphonic Acid
• 4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid
• 4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsaure
• 4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid)
• Acide 4-(2-Hydroxyethyl)Piperazine-1-Ylethanesulfonique
• Acide 4-(2-hydroxyethyl)piperazin-1-ylethanesulfonique
• Acido 4-(2-Hidroxietil)Piperazin-1-Iletanosulfonico
• Hepes
• Labotest-Bb Lt00138109
• Labotest-Bb Lt00233156
• N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic acid
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Molecular Biology Grade
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Tissue Culture Tested
• N-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid
• N-2-Hydroxyethylpiperazino-N'-2-ethanesulfonic acid
• Nsc 166663
• Piperazine, 1-(Ethylsulfonic Acid)-4- (2-Hydroxyethyl)-
• Tvz 7
• Was 13
• HEPES BUFFER
• N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANE SULPHONIC ACID
• HEPES-ACID
• 4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci
• HEPES BUFFERED SALINE
• hepesfreeacid
• GOOD'S BUFFER
• N-(2-Hydroxyethyl)piper
• HEPES BUFFER PH 7.5

2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid [Good's buffer component for biological research]

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: >99.0%(T)

Color and Shape: White to Almost white powder to crystal

Molecular weight: 238.30

Ref: 3B-H0396

HEPES, 1.0M buffer soln., pH 6.5

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Ref: 02-J61017

HEPES, 1.0M buffer soln., pH 9.0

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Ref: 02-J60440

HEPES Buffer, 0.2M buffer soln., pH 7.4, low endotoxin

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Ref: 02-J67485

HEPES, 99%

CAS: 7365-45-9

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research. This Thermo Sci

Formula: C₈H₁₈N₂O₄S

Purity: 99%

Color and Shape: White, Crystals or powder or crystalline powder

Molecular weight: 238.30

Ref: 02-A14777

HEPES, 0.5M buffer soln., pH 7.0

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Ref: 02-J60064

HEPES, 1.0M buffer soln., pH 8.0

CAS: 7365-45-9

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research. This Thermo Sci

Formula: C₈H₁₈N₂O₄S

Color and Shape: Liquid, Clear colorless

Molecular weight: 238.30

Ref: 02-J63578

HEPES, 1M Solution, pH 7.3, Molecular Biology Grade, Ultrapure

CAS: 7365-45-9

Thermo Scientific HEPES Ultrapure

Formula: C₈H₁₈N₂O₄S

Molecular weight: 238.30

Ref: 02-J16924

HEPES, 0.5M buffer soln., pH 8.0

CAS: 7365-45-9

HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. It is useful in the pH range 6.8-8.2. HEPES is used as a zwitterionic, all-purpose Good’s buffer for use in biological research. HEPES is widely used in cell culture, larg

Ref: 02-J63002

HEPES, 1.0M buffer soln., pH 7.5

CAS: 7365-45-9

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research. This Thermo Sci

Formula: C₈H₁₈N₂O₄S

Color and Shape: Liquid, Clear colorless

Molecular weight: 238.30

Ref: 02-J60712

HEPES, 0.5M buffer soln., pH 7.5

CAS: 7365-45-9

HEPES is typically used in cell culture at concentration between 5mM to 30 mM. It is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins

Ref: 02-J61275

HEPES-buffered saline, pH 7.0 (5X)

CAS: 7365-45-9

HEPES is widely used in cell culture. HEPES is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization. N-2-hydroxyethylpiperazine-N-2?-ethanesulfonic acid (Hepes) has been tested as an alternative to tris-(hydroxymethyl)aminoethane (Tris) for hydro

Formula: C₈H₁₈N₂O₄S

Color and Shape: Clear, colorless liquid

Molecular weight: 238.30

Ref: 02-J60753

HEPES, 0.5M buffer soln., pH 8.5

CAS: 7365-45-9

HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. It is useful in the pH range 6.8-8.2. HEPES is used as a zwitterionic, all-purpose Good’s buffer for use in biological research. HEPES is widely used in cell culture, larg

Ref: 02-J63218

HEPES, 0.5M buffer soln., pH 7.6

CAS: 7365-45-9

HEPES is typically used in cell culture at concentration between 5mM to 30 mM. It is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins

Ref: 02-J61047

HEPES

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: 97.0%

Color and Shape: Solid, Powder or Crystalline Powder or Solid

Molecular weight: 238.3

Ref: 10-F047861

HEPES, ≥99% (Dry Basis), Ultrapure

CAS: 7365-45-9

Thermo Scientific HEPES Ultrapure

Formula: C₈H₁₈N₂O₄S

Purity: 99%

Color and Shape: White crystalline powder

Molecular weight: 238.30

Ref: 02-J16926

HEPES-buffered saline, pH 7.0 (2X for transfection)

CAS: 7365-45-9

HEPES (2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid)-buffered saline, 7365-45-9, is a buffer used to maintain the pH of cell culture. Learn more.

Formula: C₈H₁₈N₂O₄S

Color and Shape: Clear colorless, Liquid

Molecular weight: 238.30

Ref: 02-J62623

HEPES-buffered saline, pH 6.5 (5X)

CAS: 7365-45-9

HEPES is typically used in cell culture at concentration between 5mM to 30 mM. It is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins

Formula: C₈H₁₈N₂O₄S

Molecular weight: 238.30

Ref: 02-J61239

HEPES, 1.0M buffer soln., pH 7.0

CAS: 7365-45-9

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research. This Thermo Sci

Formula: C₈H₁₈N₂O₄S

Color and Shape: Clear colorless, Liquid

Molecular weight: 238.30

Ref: 02-J62688

HEPES, 1.0M buffer soln., pH 8.5

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Ref: 02-J61360

2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: 97%

Color and Shape: Solid

Molecular weight: 238.3045

Ref: IN-DA008QEB

HEPES free acid Ultrapure

CAS: 7365-45-9

HEPES free acid Ultrapure

Formula: C₈H₁₈N₂O₄S

Purity: >99.5%

Color and Shape: white crystalline powder

Molecular weight: 238.30452g/mol

Ref: 54-BI8181

HEPES free acid Excipient

CAS: 7365-45-9

HEPES free acid Excipient

Molecular weight: 238.305g/mol

Ref: 54-BI12181

HEPES for buffer solutions

CAS: 7365-45-9

HEPES for buffer solutions

Color and Shape: Solid

Molecular weight: 238.30g/mol

Ref: 54-BIA1069

HEPES

CAS: 7365-45-9

HEPES

Purity: ≥98%

Molecular weight: 238.3g/mol

Ref: 54-BUP18543

HEPES

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: (Titration) ≥ 99.5% (dried basis)

Color and Shape: White crystalline powder

Molecular weight: 238.31

Ref: 7W-GB3086

HEPES, 99.5%, GlenCell™, suitable for cell culture

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: (Titration) 99.5 - 100.5 % (dried basis)

Color and Shape: White crystalline powder

Molecular weight: 238.31

Ref: 7W-GM5581

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Color and Shape: Neat

Molecular weight: 238.30

Ref: 04-CA14231650

HEPES

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Molecular weight: 238.30

Ref: 4Z-B-4438

HEPES

CAS: 7365-45-9

HEPES: zwitterionic buffer, stabilizes pH in cell cultures, promotes lysosome biogenesis.

Formula: C₈H₁₈N₂O₄S

Purity: 99.82%

Color and Shape: Solid

Molecular weight: 238.3

Ref: TM-T8549

HEPES buffer pH 8.0 (1M) sterile

CAS: 7365-45-9

HEPES buffer pH 8.0 (1M) sterile

Color and Shape: Liquid

Molecular weight: 238.30g/mol

Ref: 54-BIA6906

Hepes

CAS: 7365-45-9

Applications Hepes

Formula: C₈H₁₈N₂O₄S

Color and Shape: White To Off-White

Molecular weight: 238.30

Ref: TR-H998468

HEPES

CAS: 7365-45-9

HEPES, also known as 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, is a piperazinic buffer with an optimal pH range of 6.8-8.2 and a pKa of 7.48. This zwitterionic buffering agent is widely used in cell culture however when exposed to light HEPES can produce hydrogen peroxide.

Formula: C₈H₁₈N₂O₄S

Color and Shape: White Powder

Molecular weight: 238.31 g/mol

Ref: 3D-FH31182

HEPES Buffer for cell culture, 99.5%, Endotoxin (BET) 0.05EU/mg

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: min. 99.5%

Color and Shape: White, Crystalline powder, Clear, Colourless

Molecular weight: 238.30

Ref: SR-39149

HEPES Buffer for molecular biology, 99.5%

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: min. 99.5%

Color and Shape: White, Crystalline powder, Clear, Colourless

Molecular weight: 238.30

Ref: SR-16826

HEPES Buffer extrapure, 99%

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: min. 99%

Color and Shape: White, Crystalline powder, Clear, Colourless

Molecular weight: 238.30

Ref: SR-63732

HEPES Buffer for tissue culture, 99%

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: min. 99%

Color and Shape: White, Crystalline powder, Clear, Colourless

Molecular weight: 238.30

Ref: SR-48489