CAS 7365-45-9

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

Description:

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, commonly known as HEPES, is a zwitterionic buffering agent widely used in biological and biochemical research. It has a pKa value around 7.5, making it particularly effective for maintaining physiological pH in cell culture and biochemical assays. HEPES is soluble in water and exhibits good stability under physiological conditions, which is crucial for experiments requiring consistent pH levels. Its structure features a piperazine ring, a sulfonic acid group, and a hydroxyethyl side chain, contributing to its buffering capacity. HEPES is non-toxic to cells, making it suitable for various applications, including protein purification and enzyme assays. Additionally, it is less prone to interfere with biological processes compared to other buffers, such as phosphate buffers. However, it is important to note that HEPES can absorb UV light, which may affect certain spectroscopic analyses. Overall, HEPES is a versatile and widely utilized buffer in laboratory settings, particularly in the fields of molecular biology and biochemistry.

Formula: C₈H₁₈N₂O₄S

InChI: InChI=1/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)/p+1

InChI key: InChIKey=JKMHFZQWWAIEOD-UHFFFAOYSA-N

SMILES: C(CS(=O)(=O)O)N1CCN(CCO)CC1

Synonyms:

• 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
• 1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonic acid
• 2-[4-(2-Hydroxyethyl)-1-Piperazinyl]Ethanesulfonic Acid
• 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid
• 2-[4-(2-Hydroxyethyl)Piperazin-4-Ium-1-Yl]Ethanesulfonate
• 2-[4-(2-Hydroxyethyl)Piperazinediium-1-Yl]Ethanesulfonate
• 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
• 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
• 2-[4-(2-Hydroxyethyl)piperazinyl]ethanesulfonic acid
• 2-[4-(Hydroxyethyl)-1-piperazinyl]ethane sulfonic acid
• 4-(2-Hydroxyethy1)-1-piperazineethanesulfonic acid
• 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic Acid
• 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonicacid
• 4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid
• 4-(2-Hydroxyethyl)Piperazin-1-Ylethanesulphonic Acid
• 4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid
• 4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsaure
• 4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid)
• Acide 4-(2-Hydroxyethyl)Piperazine-1-Ylethanesulfonique
• Acide 4-(2-hydroxyethyl)piperazin-1-ylethanesulfonique
• Acido 4-(2-Hidroxietil)Piperazin-1-Iletanosulfonico
• Hepes
• Labotest-Bb Lt00138109
• Labotest-Bb Lt00233156
• N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic acid
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Molecular Biology Grade
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Tissue Culture Tested
• N-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid
• N-2-Hydroxyethylpiperazino-N'-2-ethanesulfonic acid
• Nsc 166663
• Piperazine, 1-(Ethylsulfonic Acid)-4- (2-Hydroxyethyl)-
• Tvz 7
• Was 13
• HEPES BUFFER
• N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANE SULPHONIC ACID
• HEPES-ACID
• 4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci
• HEPES BUFFERED SALINE
• hepesfreeacid
• GOOD'S BUFFER
• N-(2-Hydroxyethyl)piper
• HEPES BUFFER PH 7.5

2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: 97%

Color and Shape: Solid

Molecular weight: 238.3045

Ref: IN-DA008QEB

HEPES free acid Ultrapure

CAS: 7365-45-9

HEPES free acid Ultrapure

Formula: C₈H₁₈N₂O₄S

Purity: >99.5%

Color and Shape: white crystalline powder

Molecular weight: 238.30452g/mol

Ref: 54-BI8181

HEPES free acid Excipient

CAS: 7365-45-9

HEPES free acid Excipient

Molecular weight: 238.305g/mol

Ref: 54-BI12181

HEPES for buffer solutions

CAS: 7365-45-9

HEPES for buffer solutions

Color and Shape: Solid

Molecular weight: 238.30g/mol

Ref: 54-BIA1069

HEPES

CAS: 7365-45-9

HEPES

Purity: ≥98%

Molecular weight: 238.3g/mol

Ref: 54-BUP18543

HEPES

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: (Titration) ≥ 99.5% (dried basis)

Color and Shape: White crystalline powder

Molecular weight: 238.31

Ref: 7W-GB3086

HEPES, 99.5%, GlenCell™, suitable for cell culture

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: (Titration) 99.5 - 100.5 % (dried basis)

Color and Shape: White crystalline powder

Molecular weight: 238.31

Ref: 7W-GM5581

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Color and Shape: Neat

Molecular weight: 238.30

Ref: 04-CA14231650

HEPES

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Molecular weight: 238.30

Ref: 4Z-B-4438

HEPES

CAS: 7365-45-9

HEPES: zwitterionic buffer, stabilizes pH in cell cultures, promotes lysosome biogenesis.

Formula: C₈H₁₈N₂O₄S

Purity: 99.82%

Color and Shape: Solid

Molecular weight: 238.3

Ref: TM-T8549

HEPES buffer pH 8.0 (1M) sterile

CAS: 7365-45-9

HEPES buffer pH 8.0 (1M) sterile

Color and Shape: Liquid

Molecular weight: 238.30g/mol

Ref: 54-BIA6906

Hepes

CAS: 7365-45-9

Applications Hepes

Formula: C₈H₁₈N₂O₄S

Color and Shape: White To Off-White

Molecular weight: 238.30

Ref: TR-H998468

HEPES

CAS: 7365-45-9

HEPES, also known as 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, is a piperazinic buffer with an optimal pH range of 6.8-8.2 and a pKa of 7.48. This zwitterionic buffering agent is widely used in cell culture however when exposed to light HEPES can produce hydrogen peroxide.

Formula: C₈H₁₈N₂O₄S

Color and Shape: White Powder

Molecular weight: 238.31 g/mol

Ref: 3D-FH31182

HEPES Buffer for cell culture, 99.5%, Endotoxin (BET) 0.05EU/mg

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: min. 99.5%

Color and Shape: White, Crystalline powder, Clear, Colourless

Molecular weight: 238.30

Ref: SR-39149

HEPES Buffer for molecular biology, 99.5%

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: min. 99.5%

Color and Shape: White, Crystalline powder, Clear, Colourless

Molecular weight: 238.30

Ref: SR-16826

HEPES Buffer extrapure, 99%

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: min. 99%

Color and Shape: White, Crystalline powder, Clear, Colourless

Molecular weight: 238.30

Ref: SR-63732

HEPES Buffer for tissue culture, 99%

CAS: 7365-45-9

Formula: C₈H₁₈N₂O₄S

Purity: min. 99%

Color and Shape: White, Crystalline powder, Clear, Colourless

Molecular weight: 238.30

Ref: SR-48489