CAS 7365-45-9: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

Description: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, commonly known as HEPES, is a zwitterionic buffering agent widely used in biological and biochemical research. It has a pKa value around 7.5, making it particularly effective for maintaining physiological pH in cell culture and biochemical assays. HEPES is soluble in water and exhibits good stability under physiological conditions, which is crucial for experiments requiring consistent pH levels. Its structure features a piperazine ring, a sulfonic acid group, and a hydroxyethyl side chain, contributing to its buffering capacity. HEPES is non-toxic to cells, making it suitable for various applications, including protein purification and enzyme assays. Additionally, it is less prone to interfere with biological processes compared to other buffers, such as phosphate buffers. However, it is important to note that HEPES can absorb UV light, which may affect certain spectroscopic analyses. Overall, HEPES is a versatile and widely utilized buffer in laboratory settings, particularly in the fields of molecular biology and biochemistry.

Formula: C₈H₁₈N₂O₄S

InChI: InChI=1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key: InChIKey=JKMHFZQWWAIEOD-UHFFFAOYSA-N

SMILES: O=S(=O)(O)CCN1CCN(CCO)CC1

Synonyms:

• 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
• 1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonic acid
• 2-[4-(2-Hydroxyethyl)-1-Piperazinyl]Ethanesulfonic Acid
• 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid
• 2-[4-(2-Hydroxyethyl)Piperazin-4-Ium-1-Yl]Ethanesulfonate
• 2-[4-(2-Hydroxyethyl)Piperazinediium-1-Yl]Ethanesulfonate
• 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
• 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
• 2-[4-(2-Hydroxyethyl)piperazinyl]ethanesulfonic acid
• 2-[4-(Hydroxyethyl)-1-piperazinyl]ethane sulfonic acid
• 4-(2-Hydroxyethy1)-1-piperazineethanesulfonic acid
• 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic Acid
• 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonicacid
• 4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid
• 4-(2-Hydroxyethyl)Piperazin-1-Ylethanesulphonic Acid
• 4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid
• 4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsaure
• 4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid)
• Acide 4-(2-Hydroxyethyl)Piperazine-1-Ylethanesulfonique
• Acide 4-(2-hydroxyethyl)piperazin-1-ylethanesulfonique
• Acido 4-(2-Hidroxietil)Piperazin-1-Iletanosulfonico
• Hepes
• Labotest-Bb Lt00138109
• Labotest-Bb Lt00233156
• N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic acid
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Molecular Biology Grade
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Tissue Culture Tested
• N-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid
• N-2-Hydroxyethylpiperazino-N'-2-ethanesulfonic acid
• Nsc 166663
• Piperazine, 1-(Ethylsulfonic Acid)-4- (2-Hydroxyethyl)-
• Tvz 7
• Was 13

HEPES for buffer solutions

CAS: 7365-45-9

• Color and Shape: Solid
• Molecular weight: 238.30g/mol

HEPES

CAS: 7365-45-9

• Molecular weight: 238.3g/mol

HEPES, 99.5%, GlenCell™, suitable for cell culture

CAS: 7365-45-9

• Formula: C₈H₁₈N₂O₄S
• Purity: (Titration) 99.5 - 100.5 % (dried basis)
• Color and Shape: White crystalline powder
• Molecular weight: 238.31

HEPES buffer pH 8.0 (1M) sterile

CAS: 7365-45-9

• Color and Shape: Liquid
• Molecular weight: 238.30g/mol

HEPES

CAS: 7365-45-9

• Formula: C₈H₁₈N₂O₄S
• Purity: (Titration) ≥ 99.5% (dried basis)
• Color and Shape: White crystalline powder
• Molecular weight: 238.31

HEPES free acid Ultrapure

CAS: 7365-45-9

• Purity: >99.5%
• Color and Shape: white crystalline powder
• Molecular weight: 238.30g/mol

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

CAS: 7365-45-9

• Formula: C₈H₁₈N₂O₄S
• Color and Shape: Neat
• Molecular weight: 238.30

HEPES free acid, 99+%, for molecular biology, DNase, Rnase and Protease free

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. …

• Formula: C₈H₁₈N₂O₄S
• Purity: 99+%
• Color and Shape: White, Crystalline powder
• Molecular weight: 238.30

HEPES, 99%, for biochemistry

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. …

• Formula: C₈H₁₈N₂O₄S
• Purity: 99%
• Color and Shape: White, Crystalline powder or crystals
• Molecular weight: 238.30

HEPES

CAS: 7365-45-9

• Purity: 97.0%
• Color and Shape: Solid, Powder or Crystalline Powder or Solid
• Molecular weight: 238.3000030517578

HEPES

CAS: 7365-45-9

• Formula: C₈H₁₈N₂O₄S
• Molecular weight: 238.30

HEPES

CAS: 7365-45-9

HEPES: zwitterionic buffer, stabilizes pH in cell cultures, promotes lysosome biogenesis.

• Formula: C₈H₁₈N₂O₄S
• Purity: ≥98%
• Color and Shape: Solid
• Molecular weight: 238.3

Hepes

CAS: 7365-45-9

Applications Hepes

• Formula: C₈H₁₈N₂O₄S
• Color and Shape: White To Off-White
• Molecular weight: 238.30

HEPES

CAS: 7365-45-9

Organic buffering agent used in cell cultures A Good's buffer substance, pKa = 7.55 at …

• Formula: C₈H₁₈N₂O₄S
• Color and Shape: White Powder
• Molecular weight: 238.31 g/mol

HEPES Buffer for tissue culture, 99%

CAS: 7365-45-9

• Formula: C₈H₁₈N₂O₄S
• Purity: min. 99%
• Color and Shape: White, Crystalline powder, Clear, Colourless
• Molecular weight: 238.30

HEPES Buffer extrapure, 99%

CAS: 7365-45-9

• Formula: C₈H₁₈N₂O₄S
• Purity: min. 99%
• Color and Shape: White, Crystalline powder, Clear, Colourless
• Molecular weight: 238.30

HEPES Buffer for molecular biology, 99.5%

CAS: 7365-45-9

• Formula: C₈H₁₈N₂O₄S
• Purity: min. 99.5%
• Color and Shape: White, Crystalline powder, Clear, Colourless
• Molecular weight: 238.30

HEPES Buffer for cell culture, 99.5%, Endotoxin (BET) 0.05EU/mg

CAS: 7365-45-9

• Formula: C₈H₁₈N₂O₄S
• Purity: min. 99.5%
• Color and Shape: White, Crystalline powder, Clear, Colourless
• Molecular weight: 238.30