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CAS 7365-45-9

:

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

Description:
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, commonly known as HEPES, is a zwitterionic buffering agent widely used in biological and biochemical research. It has a pKa value around 7.5, making it particularly effective for maintaining physiological pH in cell culture and biochemical assays. HEPES is soluble in water and exhibits good stability under physiological conditions, which is crucial for experiments requiring consistent pH levels. Its structure features a piperazine ring, a sulfonic acid group, and a hydroxyethyl side chain, contributing to its buffering capacity. HEPES is non-toxic to cells, making it suitable for various applications, including protein purification and enzyme assays. Additionally, it is less prone to interfere with biological processes compared to other buffers, such as phosphate buffers. However, it is important to note that HEPES can absorb UV light, which may affect certain spectroscopic analyses. Overall, HEPES is a versatile and widely utilized buffer in laboratory settings, particularly in the fields of molecular biology and biochemistry.
Formula:C8H18N2O4S
InChI:InChI=1/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)/p+1
InChI key:InChIKey=JKMHFZQWWAIEOD-UHFFFAOYSA-N
SMILES:C(CS(=O)(=O)O)N1CCN(CCO)CC1
Synonyms:
  • 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
  • 1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonic acid
  • 2-[4-(2-Hydroxyethyl)-1-Piperazinyl]Ethanesulfonic Acid
  • 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid
  • 2-[4-(2-Hydroxyethyl)Piperazin-4-Ium-1-Yl]Ethanesulfonate
  • 2-[4-(2-Hydroxyethyl)Piperazinediium-1-Yl]Ethanesulfonate
  • 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
  • 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
  • 2-[4-(2-Hydroxyethyl)piperazinyl]ethanesulfonic acid
  • 2-[4-(Hydroxyethyl)-1-piperazinyl]ethane sulfonic acid
  • See more synonyms
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