CAS 7411-49-6

3,3′-Diaminobenzidine tetrahydrochloride

Description:

3,3′-Diaminobenzidine tetrahydrochloride (DAB) is an organic compound commonly used as a chromogen in immunohistochemistry and as a reagent in various chemical reactions. It appears as a dark brown to black crystalline powder and is soluble in water, which facilitates its application in biological staining. DAB is known for its ability to produce a colored precipitate upon oxidation, making it valuable for visualizing proteins and other biomolecules in tissue samples. The compound contains two amino groups, which contribute to its reactivity and ability to form stable complexes with metal ions. However, it is important to note that DAB is considered a potential carcinogen, and appropriate safety measures should be taken when handling it. In laboratory settings, it is typically used in buffered solutions and requires careful storage to maintain its stability. Overall, 3,3′-Diaminobenzidine tetrahydrochloride is a significant tool in biochemical research, particularly in the fields of pathology and molecular biology.

Formula: C₁₂H₁₄N₄·4ClH

InChI: InChI=1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key: InChIKey=KJDSORYAHBAGPP-UHFFFAOYSA-N

SMILES: NC=1C=C(C=CC1N)C2=CC(N)=C(N)C=C2.Cl

Synonyms:

• (1,1'-Biphenyl)-3,3',4,4’-Tetramine,Tetrahydrochloride
• 3,3',4,4'-Biphenyltetramine Tetrahydrochloride
• 3,3'-Diaminobenzidine Hydrochloride
• 3,3'-Diammoniumbenzidiniumtetrachloride
• 3,3,4,4-Biphenyltetramine tetrahydrochloride
• 3,3-Diaminobenzidine Chlorhydrate
• 3,3-Diaminobenzidine Isopac Of*Tetrahydrochlorid
• 3,3-Diaminobenzidine tetrahydrochloride,dihydrate
• 3,3-Diaminobenzidine*Tetrahydrochloride Tablets,
• 3,3-Diaminobezidine tetrahydrochloride
• 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride
• 3,3′-Diaminobenzidine hydrochlride
• 3,3′-Diammoniumbenzidinium tetrachloride
• 3,4,3',4'-Tetra-Aminobiphenyl Hydrochloride
• 3,4,3′,4′-Tetraaminobiphenyl tetrahydrochloride
• Biphenyl-3,3',4,4'-Tetramine Tetrahydrochloride
• Biphenyl-3,3,4,4-Tetrayltetraammonium Tetrachloride
• DAB tetrahydrochloride, dihydrate 3,3-Diaminobenzidine tetrahydrochloride, dihydrate
• Dab Liquid Substrate Dropper System,*Tru-Measure
• Dab-4Hcl
• [1,1′-Biphenyl]-3,3′,4,4′-tetramine, hydrochloride (1:4)
• [1,1′-Biphenyl]-3,3′,4,4′-tetramine, tetrahydrochloride
• 3,3-Diaminobenzidine tetrahydrochloride

3,3'-Diaminobenzidine Tetrahydrochloride Hydrate

CAS: 167684-17-5, 7411-49-6, 868272-85-9

Formula: C₁₂H₁₄N₄·4HCl·xH₂O

Purity: >98.0%(HPLC)(N)

Color and Shape: White to Amber to Dark purple powder to crystal

Molecular weight: 360.10 (as Anhydrous)

Ref: 3B-D0078

3,3'-Diaminobenzidine Tetrahydrochloride Hydrate [for Biochemical Research]

CAS: 167684-17-5, 7411-49-6, 868272-85-9

Formula: C₁₂H₁₄N₄·4HCl·xH₂O

Purity: >98.0%(HPLC)(N)

Color and Shape: White to Amber to Dark purple powder to crystal

Molecular weight: 360.10 (as Anhydrous)

Ref: 3B-D3757

3,3′-DIAMINOBENZIDINE TETRAHYDROCHLORIDE DIHYDRATE

CAS: 7411-49-6

Formula: C₁₂H₂₂Cl₄N₄O₂

Purity: 98.0%

Molecular weight: 396.13

Ref: 10-F386712

3,3’-Diaminobenzidine Tetrahydrochloride

CAS: 7411-49-6

Stability Hygroscopic
Applications A substrate for peroxidase, and a reagent for the spectrophotometric determination of selenium.

Formula: C₁₂H₁₄N₄·4ClH

Color and Shape: Neat

Molecular weight: 360.11

Ref: TR-D416150

3,3'-Diaminobenzidine tetrahydrochloride

CAS: 7411-49-6

3,3'-Diaminobenzidine tetrahydrochloride or DAB hydrochloride is the hydrochloride salt of biphenyl tetra-aniline. DAB hydrochloride is a water-soluble formulation of DAB and is used in immunohistochemical staining of nucleic acids and proteins. DAB hydrochloride is used in Alzheimer's disease research, Aβ protein amyloid plaques are targeted by a primary antibody, and subsequently by a secondary antibody, which is conjugated with a peroxidase enzyme. This will bind DAB as a substrate and oxidize causing a colour change. 3,3’-Diaminobenzidine tetrahydrochloride is also available as the tetrahydrochloride hydrate and as the free base.

Formula: C₁₂H₁₄N₄•4HCl

Purity: Min. 95%

Color and Shape: Off-White Powder

Molecular weight: 360.12 g/mol

Ref: 3D-FD11034