CAS 74551-31-8

D-val-phe-lys P-nitroanilide*dihydrochloride

Description:

D-Val-Phe-Lys P-nitroanilide dihydrochloride is a synthetic peptide substrate commonly used in biochemical assays, particularly for the study of proteolytic enzymes. This compound features a sequence of amino acids, specifically D-valine, phenylalanine, and lysine, linked to a p-nitroanilide moiety, which serves as a chromogenic indicator. Upon cleavage by specific proteases, the p-nitroanilide is released, resulting in a measurable color change that can be quantified spectrophotometrically. The dihydrochloride form indicates the presence of two hydrochloride ions, which can enhance solubility in aqueous solutions. This compound is typically utilized in research settings to investigate enzyme kinetics and substrate specificity. Its stability and reactivity make it a valuable tool in enzymology, particularly in the study of serine proteases. Safety precautions should be observed when handling this compound, as with many chemical substances, due to potential irritant properties.

Formula: C₂₆H₃₈Cl₂N₆O₅

InChI: InChI=1/C26H36N6O5.2ClH/c1-17(2)23(28)26(35)31-22(16-18-8-4-3-5-9-18)25(34)30-21(10-6-7-15-27)24(33)29-19-11-13-20(14-12-19)32(36)37;;/h3-5,8-9,11-14,17,21-23H,6-7,10,15-16,27-28H2,1-2H3,(H,29,33)(H,30,34)(H,31,35);2*1H/t21-,22-,23+;;/m0../s1