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CAS 76-54-0

:

2′,7′-Dichlorofluorescein

Description:
2′,7′-Dichlorofluorescein, with the CAS number 76-54-0, is a synthetic organic compound widely used as a fluorescent dye and pH indicator. It exhibits a bright green fluorescence when excited by ultraviolet or blue light, making it valuable in various biological and chemical applications, including microscopy and flow cytometry. The compound has a molecular formula of C13H8Cl2O5 and features two chlorine atoms substituted at the 2' and 7' positions of the fluorescein structure, which enhances its photostability and solubility in aqueous solutions. 2′,7′-Dichlorofluorescein is sensitive to pH changes, displaying distinct fluorescence intensity variations across different pH levels, which allows for its use in monitoring cellular environments. Additionally, it is non-toxic at low concentrations, making it suitable for biological studies. However, like many chemical substances, it should be handled with care, following appropriate safety protocols to avoid exposure.
Formula:C20H10Cl2O5
InChI:InChI=1/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)27-18-8-16(24)14(22)6-12(18)19(11)9-3-1-2-4-10(9)20(25)26/h1-8,23H,(H,25,26)
InChI key:InChIKey=VFNKZQNIXUFLBC-UHFFFAOYSA-N
SMILES:O=C1OC2(C=3C(OC=4C2=CC(Cl)=C(O)C4)=CC(O)=C(Cl)C3)C=5C1=CC=CC5
Synonyms:
  • Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, 2′,7′-dichloro-3′,6′-dihydroxy-
  • D and C Orange No. 25
  • 2′,7′-Dichlorofluorescein
  • Fluorescein, 2′,7′-dichloro-
  • 2′,7′-Dichloro-3′,6′-dihydroxyspiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
  • FLUORESCEIN 27
  • DICHLOROFLUORESCEIN TS
  • 2',7'-DICHLORO-FLUORESCEINE
  • 2',7'-DICHLORO-3,6-FLUORANDIOL
  • 2-(2,7-dichloro-6-hydroxy-3-oxo-3-H-xanthen-9-yl)benzoic acid
  • See more synonyms
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