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CAS 80265-04-9

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4-methylumbelliferyl N-acetyl-A-D-glucosaminide

Description:
4-Methylumbelliferyl N-acetyl-α-D-glucosaminide is a synthetic substrate commonly used in biochemical assays, particularly for the detection of enzymes such as N-acetyl-β-D-glucosaminidase. This compound features a 4-methylumbelliferone moiety, which is a fluorescent marker, allowing for easy visualization of enzymatic activity. The N-acetyl-α-D-glucosaminide part of the molecule serves as the glycosidic substrate. Upon hydrolysis by the target enzyme, the release of 4-methylumbelliferone results in a measurable increase in fluorescence, making it a valuable tool in research and diagnostic applications. The compound is typically soluble in organic solvents and water, and its fluorescence properties can be influenced by pH and solvent conditions. It is important to handle this substance with care, following appropriate safety protocols, as with any chemical reagent. Overall, 4-methylumbelliferyl N-acetyl-α-D-glucosaminide is a crucial compound in enzymology and molecular biology for studying glycosidase activity.
Formula:C18H21NO8
Synonyms:
  • 4-Methylumbelliferyl 2-Acetamido-2-Deoxy-a-D-Glucopyranoside
  • 4-Methylumbelliferyl-2-Acetamido-2-Deoxy-Alpha-D-Glucopyranoside
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