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CAS 83809-73-8

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o-Tolyltetrazolium Red Formazan

Description:
o-Tolyltetrazolium Red Formazan, with the CAS number 83809-73-8, is a chemical compound commonly used in biochemical assays, particularly in cell viability and proliferation studies. It is a derivative of tetrazolium salts, which are reduced by viable cells to form a colored formazan product, allowing for quantification of metabolic activity. This compound typically exhibits a deep red color, which can be measured spectrophotometrically, making it useful for various laboratory applications. The solubility of o-Tolyltetrazolium Red Formazan is generally in organic solvents, and it is relatively stable under standard laboratory conditions. Its use is prevalent in assays such as the MTT assay, where it serves as an indicator of cellular health and proliferation. Additionally, the compound's ability to form formazan crystals can provide insights into cellular processes and responses to treatments. Safety precautions should be observed when handling this compound, as with all chemical substances, to minimize exposure and ensure proper laboratory practices.
Formula:C20H18N4
InChI:InChI=1/C20H18N4/c1-16-10-8-9-15-19(16)22-24-20(17-11-4-2-5-12-17)23-21-18-13-6-3-7-14-18/h2-15,21H,1H3/b23-20-,24-22+
Synonyms:
  • 3,5-Diphenyl-1-(o-tolyl)formazan
  • (E)-1-(2-methylphenyl)-2-[(Z)-phenyl(phenylhydrazono)methyl]diazene
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