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CAS 84325-23-5

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4-methylumbelliferyl-beta-D-lactoside

Description:
4-Methylumbelliferyl-beta-D-lactoside (MUL) is a synthetic substrate commonly used in biochemical assays, particularly for the detection of β-galactosidase activity. It is characterized by its fluorescent properties, which are activated upon hydrolysis by β-galactosidase, resulting in the release of 4-methylumbelliferone, a compound that emits blue fluorescence when excited by ultraviolet light. This makes MUL a valuable tool in molecular biology and microbiology for monitoring enzyme activity and studying gene expression. The compound is typically soluble in water and organic solvents, allowing for versatile applications in laboratory settings. Its structure includes a lactoside moiety, which is a disaccharide composed of glucose and galactose, linked to the fluorescent 4-methylumbelliferone. The use of MUL in assays is favored due to its sensitivity and the ease of detecting the fluorescent product, making it an essential reagent in various research and diagnostic applications. Safety data sheets should be consulted for handling and storage guidelines, as with any chemical substance.
Formula:C22H28O13
InChI:InChI=1/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15+,16+,17-,18-,19-,20-,21-,22+/m1/s1
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