5-(AND-6)-Chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, commonly referred to as DCFDA, is a fluorescent probe used primarily for detecting reactive oxygen species (ROS) in biological systems. This compound is a non-fluorescent diacetate derivative that becomes fluorescent upon deacetylation and oxidation, allowing for sensitive detection of oxidative stress in cells. Its structure features a chloromethyl group and dichlorinated dihydrofluorescein moiety, contributing to its fluorescence properties. The diacetate groups enhance cell permeability, facilitating its uptake into living cells. Once inside, esterases cleave the acetate groups, converting it to the active form that fluoresces upon oxidation. DCFDA is widely utilized in various fields, including cell biology, pharmacology, and toxicology, to study oxidative stress and related cellular processes. The compound is typically characterized by its absorption and emission spectra, which are crucial for fluorescence microscopy and flow cytometry applications. Safety data sheets should be consulted for handling and disposal guidelines, as with any chemical substance.

5-(AND-6)-CHLOROMETHYL-2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE

850013-49-9: 5-(AND-6)-Chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, commonly referred to as DCFDA, is a fluorescent probe used primarily for detecting reactive oxygen species (ROS) in biological systems. This compound is a non-fluorescent diacetate derivative that becomes fluorescent upon deacetylation and oxidation, allowing for sensitive detection of oxidative stress in cells. Its structure features a chloromethyl group and dichlorinated dihydrofluorescein moiety, contributing to its fluorescence properties. The diacetate groups enhance cell permeability, facilitating its uptake into living cells. Once inside, esterases cleave the acetate groups, converting it to the active form that fluoresces upon oxidation. DCFDA is widely utilized in various fields, including cell biology, pharmacology, and toxicology, to study oxidative stress and related cellular processes. The compound is typically characterized by its absorption and emission spectra, which are crucial for fluorescence microscopy and flow cytometry applications. Safety data sheets should be consulted for handling and disposal guidelines, as with any chemical substance.

<p>CM-H2DCFDA is a fluorescent indicator for reactive oxygen species (ROS) in cells.</p>

CAS 850013-49-9

5-(AND-6)-CHLOROMETHYL-2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE

CM-H2DCFDA

Ref: TM-T30991