CAS 85901-57-1

Pyr-Phe-Leu-pNA

Description:

Pyr-Phe-Leu-pNA, also known as Pyridyl Phenyl Leucine p-Nitroanilide, is a synthetic peptide substrate commonly used in biochemical assays, particularly in the study of proteolytic enzymes. This compound features a pyridine group, a phenylalanine residue, a leucine residue, and a p-nitroanilide moiety, which collectively contribute to its properties as a substrate for various proteases. The presence of the p-nitroanilide group allows for colorimetric detection upon cleavage by enzymes, making it useful in enzyme activity assays. Pyr-Phe-Leu-pNA is typically soluble in organic solvents and exhibits stability under standard laboratory conditions. Its molecular structure enables specific interactions with target enzymes, facilitating the study of enzyme kinetics and mechanisms. Additionally, due to its synthetic nature, it can be modified to enhance specificity or sensitivity for particular applications in research and diagnostics. As with all chemical substances, proper handling and safety precautions should be observed when working with Pyr-Phe-Leu-pNA in laboratory settings.

Formula: C₂₆H₃₁N₅O₆

InChI: InChI=1/C26H31N5O6/c1-16(2)14-21(25(34)27-18-8-10-19(11-9-18)31(36)37)29-26(35)22(15-17-6-4-3-5-7-17)30-24(33)20-12-13-23(32)28-20/h3-11,16,20-22H,12-15H2,1-2H3,(H,27,34)(H,28,32)(H,29,35)(H,30,33)/t20-,21-,22-/m0/s1

SMILES: CC(C)C[C@@H](C(=O)Nc1ccc(cc1)N(=O)=O)N=C([C@H](Cc1ccccc1)N=C([C@@H]1CCC(=N1)O)O)O

Synonyms:

• Pyroglutamyl-phenylalanyl-leucine-4-nitroanilide
• Pglu-phe-leu-pna
• L-Leucinamide, 5-oxo-L-prolyl-L-phenylalanyl-N-(4-nitrophenyl)-
• 5-oxo-L-prolyl-L-phenylalanyl-N-(4-nitrophenyl)-L-leucinamide
• pglu-phe-leu P-nitroanilide
• PYR-FL-PNA
• PYR-PHE-LEU-PNA
• PYR-PHE-GLY-PNA
• Pyr-FG-pNA
• L-PYROGLUTAMYL-L-PHENYLALANYL-L-LEUCINE P-NITROANILIDE

Pyr-Phe-Leu-pNA

CAS: 85901-57-1

Chromogenic substrate for thiol proteinases from plants (caricain, papain, ficin, and bromelain) and from mammalian brain (cathepsin B). pEFL-pNA is also cleaved by a serine carboxypeptidase (HlSCP1) identified in the midgut of the hard tick Haemaphysalis longicornis, which was shown to process hemoglobin.

Formula: C₂₆H₃₁N₅O₆

Purity: 99.4%

Color and Shape: Light Yellow Powder

Molecular weight: 509.56

Ref: 01-4010747

Pyr-Phe-Leu-pNA

CAS: 85901-57-1

Pyr-Phe-Leu-pNA is a proteolytic enzyme that can be used as a pharmaceutical drug. It has been shown to have insecticidal activity against the Anopheles mosquito and other insects, as well as antimicrobial activity against bacteria and fungi. Pyr-Phe-Leu-pNA is activated by tryptophan fluorescence, which is why it is suitable for use in peptide mapping. This enzyme also has an important physiological function in the zymogen activation pathway, which enables it to act as an enzyme substrate. Pyr-Phe-Leu-pNA will also degrade proteins that contain sequences of leishmania protease activity or signal peptide.

Formula: C₂₆H₃₁N₅O₆

Purity: Min. 95%

Molecular weight: 509.55 g/mol

Ref: 3D-SPL-3130

Pyr-Phe-Leu-pNA

CAS: 85901-57-1

Pyr-Phe-Leu-pNA is a proteolytic enzyme that is used in the production of monoclonal antibodies. The enzyme was originally isolated from human pancreas, but has also been found in other sources including eggs, bovine pancreas, and various bacteria. Pyr-Phe-Leu-pNA hydrolyzes peptide bonds with a preference for serine and threonine residues. This enzyme has been shown to be effective at cleaving influenza virus protein hemagglutinin, which may be useful in the development of new vaccines. Pyr-Phe-Leu-pNA has also been shown to have high salt tolerance, making it a good candidate for use in food processing applications.

Formula: C₂₆H₃₁N₅O₆

Purity: Min. 95%

Molecular weight: 509.55 g/mol

Ref: 3D-FP110746