CAS 897-83-6

Diethylumbelliferyl phosphate

Description:

Diethylumbelliferyl phosphate (CAS 897-83-6) is an organophosphate compound commonly used as a substrate in biochemical assays, particularly for the detection of phosphatase activity. It is characterized by its ability to release a fluorescent product upon hydrolysis, making it valuable in various biochemical and analytical applications. The compound features a diethyl phosphate group attached to an umbelliferone moiety, which contributes to its fluorescent properties. Diethylumbelliferyl phosphate is typically a colorless to pale yellow solid, soluble in organic solvents, and exhibits stability under standard laboratory conditions. Its use is prevalent in enzymatic studies, where it serves as a sensitive indicator for enzyme activity, particularly in the context of research involving phosphatases. Safety precautions should be taken when handling this compound, as it may pose health risks typical of organophosphates, including potential neurotoxicity. Overall, diethylumbelliferyl phosphate is a significant tool in biochemical research, facilitating the study of enzyme kinetics and mechanisms.

Formula: C₁₄H₁₇O₆P

InChI: InChI=1/C14H17O6P/c1-4-17-21(16,18-5-2)20-11-6-7-12-10(3)8-14(15)19-13(12)9-11/h6-9H,4-5H2,1-3H3

InChI key: InChIKey=GHGRYKAFUNNHBG-UHFFFAOYSA-N

SMILES: CC=1C=2C(=CC(OP(OCC)(OCC)=O)=CC2)OC(=O)C1

Synonyms:

• Coumarin, 7-hydroxy-4-methyl-, diethyl phosphate
• Diethylumbelliferyl phosphate
• Phosphoric acid, diethyl 4-methyl-2-oxo-2H-1-benzopyran-7-yl ester
• 7-hydroxy-4-methyl-coumaridiethylphosphate
• DEUP, UBP

Diethylumbelliferyl phosphate

CAS: 897-83-6

Formula: C₁₃H₁₅O₆P

Purity: 98%

Molecular weight: 298.2284

Ref: IN-DA003ZWM

Diethylumbelliferyl phosphate

CAS: 897-83-6

Formula: C₁₄H₁₇O₆P

Molecular weight: 312.25

Ref: 7W-GL4927

Diethylumbelliferyl phosphate

CAS: 897-83-6

Diethylumbelliferyl phosphate (DEUP) is a potent and selective inhibitor of cholesterol esterase that does not inhibit protein kinase A activity in vitro. It disrupts steroidogenesis by blocking cholesterol transport to mitochondria in steroidogenic cells, with an IC50 of 11.6 μM. This compound may also limit the absorption of dietary cholesterol.

Formula: C₁₄H₁₇O₆P

Color and Shape: Solid

Molecular weight: 312.25

Ref: TM-T201163