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CAS 897-83-6

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Diethylumbelliferyl phosphate

Description:
Diethylumbelliferyl phosphate (CAS 897-83-6) is an organophosphate compound commonly used as a substrate in biochemical assays, particularly for the detection of phosphatase activity. It is characterized by its ability to release a fluorescent product upon hydrolysis, making it valuable in various biochemical and analytical applications. The compound features a diethyl phosphate group attached to an umbelliferone moiety, which contributes to its fluorescent properties. Diethylumbelliferyl phosphate is typically a colorless to pale yellow solid, soluble in organic solvents, and exhibits stability under standard laboratory conditions. Its use is prevalent in enzymatic studies, where it serves as a sensitive indicator for enzyme activity, particularly in the context of research involving phosphatases. Safety precautions should be taken when handling this compound, as it may pose health risks typical of organophosphates, including potential neurotoxicity. Overall, diethylumbelliferyl phosphate is a significant tool in biochemical research, facilitating the study of enzyme kinetics and mechanisms.
Formula:C14H17O6P
InChI:InChI=1/C14H17O6P/c1-4-17-21(16,18-5-2)20-11-6-7-12-10(3)8-14(15)19-13(12)9-11/h6-9H,4-5H2,1-3H3
InChI key:InChIKey=GHGRYKAFUNNHBG-UHFFFAOYSA-N
SMILES:CC=1C=2C(=CC(OP(OCC)(OCC)=O)=CC2)OC(=O)C1
Synonyms:
  • Coumarin, 7-hydroxy-4-methyl-, diethyl phosphate
  • Diethylumbelliferyl phosphate
  • Phosphoric acid, diethyl 4-methyl-2-oxo-2H-1-benzopyran-7-yl ester
  • See more synonyms
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