CAS 90052-53-2

1-Piperazineethanesulfonic acid

Description:

1-Piperazineethanesulfonic acid, commonly referred to as PIPES, is a zwitterionic buffering agent widely used in biological and biochemical research. It is characterized by its ability to maintain a stable pH in solutions, typically within the range of 6.1 to 7.5, making it particularly useful in cell culture and protein studies. PIPES is soluble in water and exhibits low toxicity, which enhances its suitability for various laboratory applications. The compound features a piperazine ring, which contributes to its buffering capacity, and a sulfonic acid group that enhances its solubility. Additionally, PIPES is stable under a variety of conditions, including temperature fluctuations and the presence of divalent cations, which can affect other buffering agents. Its chemical structure allows it to interact effectively with biological macromolecules, making it a preferred choice for experiments involving enzymes and nucleic acids. Overall, PIPES is valued for its effectiveness, stability, and minimal interference with biological systems.

Formula: C₆H₁₄N₂O₃S