Deoxyribonuclease II (DNase II) is an endonuclease enzyme that plays a crucial role in the degradation of DNA, particularly during cellular processes such as apoptosis and the clearance of cellular debris. It is classified as a metalloenzyme, requiring divalent metal ions, such as magnesium or manganese, for its enzymatic activity. DNase II is primarily found in lysosomes, where it facilitates the breakdown of extracellular DNA and contributes to the immune response by processing apoptotic cells. The enzyme exhibits a preference for double-stranded DNA and is known for its ability to cleave DNA into oligonucleotides. Its activity is pH-dependent, typically functioning optimally in acidic conditions. DNase II is distinct from other DNases, such as DNase I, in its substrate specificity and cellular localization. The enzyme is also of interest in various biotechnological applications, including molecular biology techniques and potential therapeutic uses in gene therapy and cancer treatment. Understanding its characteristics and mechanisms can provide insights into cellular processes and disease states.

Deoxyribonuclease II

9025-64-3: Deoxyribonuclease II (DNase II) is an endonuclease enzyme that plays a crucial role in the degradation of DNA, particularly during cellular processes such as apoptosis and the clearance of cellular debris. It is classified as a metalloenzyme, requiring divalent metal ions, such as magnesium or manganese, for its enzymatic activity. DNase II is primarily found in lysosomes, where it facilitates the breakdown of extracellular DNA and contributes to the immune response by processing apoptotic cells. The enzyme exhibits a preference for double-stranded DNA and is known for its ability to cleave DNA into oligonucleotides. Its activity is pH-dependent, typically functioning optimally in acidic conditions. DNase II is distinct from other DNases, such as DNase I, in its substrate specificity and cellular localization. The enzyme is also of interest in various biotechnological applications, including molecular biology techniques and potential therapeutic uses in gene therapy and cancer treatment. Understanding its characteristics and mechanisms can provide insights into cellular processes and disease states.

<p>Deoxyribonuclease II (DNase II, deoxyribonucleate 3'-nucleotidohydrolase, acid deoxyribonuclease, acid DNase, EC 3.1.22.1) is an endonuclease that cleaves DNA, yielding 3'-phosphate-terminated polynucleotides with a free hydroxyl group on position 5'. One unit of the DNase II will increase the absorbance of 260nm light at a rate of 0.001/minute in 1 ml reaction volume at pH 4.6 and 25°C.</p>

CAS 9025-64-3

Deoxyribonuclease II

Deoxyribonuclease II from porcine spleen

Ref: 3D-JAA02564