Thermolysin is a heat-stable proteolytic enzyme classified as a metalloprotease, primarily derived from the bacterium *Bacillus thermoproteolyticus*. It is characterized by its ability to catalyze the hydrolysis of peptide bonds in proteins, particularly at the carboxyl side of large hydrophobic amino acids. This enzyme exhibits optimal activity at elevated temperatures, typically around 70°C, which makes it particularly useful in industrial applications that require high-temperature processes, such as in the food industry for protein modification and in biotechnological applications for peptide synthesis. Thermolysin is also notable for its requirement of divalent metal ions, such as zinc, for its enzymatic activity, which plays a crucial role in its structural stability and catalytic function. Additionally, it has a broad substrate specificity, allowing it to act on various protein substrates, making it valuable in research and commercial applications. Its stability and activity under extreme conditions distinguish it from other proteases, contributing to its utility in various biochemical and industrial processes.

Thermolysin

9073-78-3: Thermolysin is a heat-stable proteolytic enzyme classified as a metalloprotease, primarily derived from the bacterium *Bacillus thermoproteolyticus*. It is characterized by its ability to catalyze the hydrolysis of peptide bonds in proteins, particularly at the carboxyl side of large hydrophobic amino acids. This enzyme exhibits optimal activity at elevated temperatures, typically around 70°C, which makes it particularly useful in industrial applications that require high-temperature processes, such as in the food industry for protein modification and in biotechnological applications for peptide synthesis. Thermolysin is also notable for its requirement of divalent metal ions, such as zinc, for its enzymatic activity, which plays a crucial role in its structural stability and catalytic function. Additionally, it has a broad substrate specificity, allowing it to act on various protein substrates, making it valuable in research and commercial applications. Its stability and activity under extreme conditions distinguish it from other proteases, contributing to its utility in various biochemical and industrial processes.

Thermolysin is an extracellular metalloendopeptidase from Bacillus thermolysinophilus that selectively catalyzes short peptides.

CAS 9073-78-3

Thermolysin

Thermolysin

Ref: TM-T76009