CAS 911-77-3

N-α-Benzoyl-DL-arginine-p-nitroanilide hydrochloride

Description:

N-α-Benzoyl-DL-arginine-p-nitroanilide hydrochloride, commonly referred to as BAPNA, is a synthetic compound primarily used as a substrate in biochemical assays to study proteolytic enzymes, particularly serine proteases. This compound features a benzoyl group attached to the amino acid arginine, with a p-nitroanilide moiety that serves as a chromogenic indicator. Upon enzymatic cleavage by proteases, BAPNA releases p-nitroaniline, which can be quantitatively measured due to its distinct color change, making it a valuable tool in enzyme kinetics studies. BAPNA is typically a white to off-white crystalline powder, soluble in organic solvents and slightly soluble in water. Its molecular structure contributes to its stability and reactivity, allowing for effective monitoring of enzyme activity. Additionally, as a hydrochloride salt, it exhibits enhanced solubility in aqueous solutions, facilitating its use in various laboratory applications. Proper handling and storage conditions are essential to maintain its integrity and effectiveness in experimental settings.

Formula: C₁₉H₂₂N₆O₄·ClH

InChI: InChI=1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H

InChI key: InChIKey=DEOKFPFLXFNAON-UHFFFAOYSA-N

SMILES: N(C(C(NC(=O)C1=CC=CC=C1)CCCNC(=N)N)=O)C2=CC=C(N(=O)=O)C=C2.Cl

Synonyms:

• Benzamide, N-[4-[(aminoiminomethyl)amino]-1-[[(4-nitrophenyl)amino]carbonyl]butyl]-, hydrochloride (1:1)
• Benzamide, N-[4-[(aminoiminomethyl)amino]-1-[[(4-nitrophenyl)amino]carbonyl]butyl]-, monohydrochloride
• N-Benzoyl-Dl-Arginine-4-Nitroanilide Hydrochloride
• N-α-Benzoyl-<span class="text-smallcaps">D</smallcap><smallcap>L</span>-arginine-p-nitroanilide hydrochloride
• N^α-Benzoyl-<span class="text-smallcaps">D</smallcap><smallcap>L</span>-arginine-p-nitroanilide hydrochloride
• NSC 83264
• Valeranilide, 2-benzamido-5-guanidino-4′-nitro-, monohydrochloride, <span class="text-smallcaps">DL</span>-
• Nα-Benzoyl-DL-arginine-p-nitroanilide hydrochloride
• Valeranilide, 2-benzamido-5-guanidino-4′-nitro-, monohydrochloride, DL-

Bz-DL-Arg-pNA · HCl

CAS: 911-77-3

Substrate for trypsin, papain, acrosin, plasmin, cathepsin B, and cathepsin H.

Formula: C₁₉H₂₂N₆O₄·HCl

Purity: ≥ 99%

Color and Shape: Light Yellow

Molecular weight: 434.88

Ref: 01-4001028

Benzamide, N-​[4-​[(aminoiminomethyl)​amino]​-​1-​[[(4-​nitrophenyl)​amino]​carbonyl]​butyl]​-​, hydrochloride (1:1)

CAS: 911-77-3

Formula: C₁₉H₂₃ClN₆O₄

Purity: 98%

Color and Shape: Solid

Molecular weight: 434.8767

Ref: IN-DA00IGHF

N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride

CAS: 911-77-3

N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride

Formula: C₁₉H₂₂N₆O₄·ClH

Color and Shape: Pale Yellow Powder

Molecular weight: 434.88g/mol

Ref: 54-BIB1056

N-Benzoyl-DL-arginine 4-nitroanilide hydrochloride

CAS: 911-77-3

N-α-benzoyl-DL-arginine-4-nitroanilide hydrochloride is commonly used as a substrate in various enzymatic assays, particularly for studying proteases like trypsin and chymotrypsin. It serves as a synthetic substrate to measure the activity of these enzymes. For instance, it has been used to determine trypsin activity in different organisms and tissues. The substrate has also been employed in studying the enzymatic properties of various proteases, including those found in latex, snake venoms,  and plant seeds. Additionally, it has been utilized to assess the effects of different compounds on trypsin activity, such as condensed tannins and wine.

Formula: C₁₉H₂₂N₆O₄•HCl

Purity: Min. 95%

Color and Shape: Powder

Molecular weight: 434.88 g/mol

Ref: 3D-EB32011

N-a-Benzoyl-DL-Arginine-4-Nitroanilide Hydrochloride (BANI, L-BAPNA) extrapure, 98%

CAS: 911-77-3

Formula: C₁₉H₂₂N₆O₄·HCl

Purity: min. 98%

Color and Shape: Yellow, Powder

Molecular weight: 434.89

Ref: SR-96249

N-(5-Guanidino-1-((4-nitrophenyl)amino)-1-oxopentan-2-yl)benzamide hydrochloride

CAS: 911-77-3

Formula: C₁₉H₂₃ClN₆O₄

Purity: 97%

Molecular weight: 434.88

Ref: 10-F544662

Bz-DL-Arg-pNA • HCl [DL-BAPA]

CAS: 911-77-3

Bz-DL-Arg-pNA • HCl [DL-BAPA] is a trypsin and trypsin-like protease substrate. It is used as a tool in biochemical studies to investigate the degradation of proteins by these enzymes. Bz-DL-Arg-pNA • HCl [DL-BAPA] is also used as an experimental model for diseases that affect enzyme activity, such as rheumatoid arthritis and multiple sclerosis.

Formula: C₁₉H₂₂N₆O₄•HCl

Purity: Min. 95%

Molecular weight: 434.88 g/mol

Ref: 3D-SRN-3013