CAS 92557-80-7

5-Carboxyfluorescein-N-hydroxysuccinimide ester

Description:

5-Carboxyfluorescein-N-hydroxysuccinimide ester is a fluorescent dye commonly used in bioconjugation and labeling applications in biochemical research. This compound features a carboxyfluorescein moiety, which provides strong fluorescence properties, making it suitable for various imaging techniques. The N-hydroxysuccinimide (NHS) ester group enhances the reactivity of the molecule, allowing it to form stable amide bonds with primary amines in proteins, peptides, or other biomolecules. This characteristic is particularly valuable for creating fluorescently labeled conjugates for assays, flow cytometry, and microscopy. The compound is typically soluble in organic solvents and exhibits stability under appropriate storage conditions. Its fluorescence can be excited by specific wavelengths, making it useful for multiplexing applications. However, care must be taken to avoid hydrolysis of the NHS ester in aqueous environments, which can lead to loss of reactivity. Overall, 5-Carboxyfluorescein-N-hydroxysuccinimide ester is a versatile tool in molecular biology and biochemistry for tracking and visualizing biomolecules.

Formula: C₂₅H₁₅NO₉

InChI: InChI=1/C25H15NO9/c27-13-2-5-17-19(10-13)33-20-11-14(28)3-6-18(20)25(17)16-4-1-12(9-15(16)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2

SMILES: c1cc2c(cc1C(=O)ON1C(=O)CCC1=O)C(=O)OC12c2ccc(cc2Oc2cc(ccc12)O)O

Synonyms:

• 5-Carboxyfluorescein, succinimidyl ester
• 1-{[(3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-5-yl)carbonyl]oxy}pyrrolidine-2,5-dione
• 5-Carboxyfluorescein N-succinimidyl ester
• 5-Fam Se
• 5-CARBOXYFLUORESCEIN-N-HYDROXYSUCCINIMIDE ESTER
• carboxyfluorescein-NHS ester
• 5-CARBOXYFLUORESCEIN SE
• 1-[[36Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9[9H]xanthen]-5-yl)carbonyl]oxy]-2,5-pyrrolidinedione
• 5(6)-CARBOXYFLUORESCEIN-NHS
• 5-CARBOXYFLUORESCEIN SUCCINIMIDE ESTER
• 5-FAM-NHS
• 5-CARBOXYFLUORESCEIN-NHS

5-Carboxyfluorescein N-Succinimidyl Ester

CAS: 92557-80-7

Formula: C₂₅H₁₅NO₉

Purity: >90.0%(HPLC)

Color and Shape: Light yellow to Yellow to Orange powder to crystal

Molecular weight: 473.39

Ref: 3B-C2479

5-Carboxyfluorescein N-Succinimidyl Este

CAS: 92557-80-7

Formula: C₂₅H₁₅NO₉

Purity: 90%

Color and Shape: Solid

Molecular weight: 473.3879

Ref: IN-DA00348N

5-FAM SE

CAS: 92557-80-7

5-FAM SE

Purity: ≥95%

Molecular weight: 473.39g/mol

Ref: 54-BUP06131

5-FAM SE

CAS: 92557-80-7

5-FAM SE, a single-isomer green fluorophore, labels peptides, proteins, nucleotides.

Formula: C₂₅H₁₅NO₉

Purity: 96.19% - 98.49%

Color and Shape: Yellow To Orange Solid

Molecular weight: 473.39

Ref: TM-T14049

5-Carboxyfluorescein,succinimidyl ester

CAS: 92557-80-7

Ref: 7W-GK4616

5-Carboxyfluorescein N-hydroxy succinimide ester

CAS: 92557-80-7

5-Carboxyfluorescein N-hydroxy succinimide ester

Purity: 98%

Color and Shape: Pale Yellow Powder

Molecular weight: 473.39g/mol

Ref: 54-BIC1054

5-Carboxyfluorescein-N-hydroxysuccinimide Ester

Controlled Product

CAS: 92557-80-7

Applications 5-Carboxyfluorescein-N-hydroxysuccinimide Ester is one of the most popular green fluorescent probe used for labeling peptides, proteins and nucleotides. It was used as a reagent in the determination of the intracellular pH in bacteria.
References Breeuwer, P., et al.: Appl. Environ. Microbiol., 62, 178 (1996);

Formula: C₂₅H₁₅NO₉

Color and Shape: Neat

Molecular weight: 473.39

Ref: TR-C178350

5-Carboxyfluorescein-N-hydroxysuccinimide ester

CAS: 92557-80-7

5-Carboxyfluorescein(5-FAM) is a self-quenching dye and one of the most common labelling agents for biomolecules, cell staining and localisation studies. The fluorescence is pH dependent, therefore it has been used to measure the internal pH of cells. As it is only membrane permeable to dead cells, it can be used as a staining agent to distinguish viable and non-viable cells. It is also used in PCR to label the 5’ end of oligonucleotides in the presence of a quencher at the 3’ end (dual labelled probe). During the amplification process, the dye is cleaved and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. The carboxylic acid can be activated to react with a primary amine.

Formula: C₂₅H₁₅NO₉

Purity: Min. 95 Area-%

Color and Shape: White Powder

Molecular weight: 473.39 g/mol

Ref: 3D-FC19777

5-Carboxyfluorescein N-Succinimidyl Ester

CAS: 92557-80-7

Formula: C₂₅H₁₅NO₉

Purity: ≥90%

Color and Shape: Solid

Molecular weight: 473.393

Ref: 10-F546112