CAS 92557-81-8

6-Carboxyfluorescein succinimidyl ester

Description:

6-Carboxyfluorescein succinimidyl ester (CAS 92557-81-8) is a fluorescent dye commonly used in bioconjugation and labeling applications in biochemical research. This compound features a carboxyfluorescein moiety, which provides strong fluorescence properties, making it suitable for various imaging and detection techniques. The succinimidyl ester functional group allows for the efficient conjugation to amine-containing biomolecules, such as proteins and peptides, facilitating the study of biological processes. The dye exhibits high stability and solubility in aqueous solutions, which is advantageous for in vitro experiments. Its fluorescence can be excited by light in the visible spectrum, typically around 490 nm, and emits light at approximately 520 nm, making it useful for applications in flow cytometry, microscopy, and other fluorescence-based assays. Additionally, the presence of the carboxylic acid group enhances its compatibility with biological systems, allowing for effective labeling without significantly altering the properties of the target molecules. Overall, 6-Carboxyfluorescein succinimidyl ester is a versatile tool in molecular biology and biochemistry for tracking and visualizing biomolecules.

Formula: C₂₅H₁₅NO₉

InChI: InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2

InChI key: InChIKey=VDABVNMGKGUPEY-UHFFFAOYSA-N

SMILES: O=C1OC2(C=3C1=CC=C(C(ON4C(=O)CCC4=O)=O)C3)C=5C(OC=6C2=CC=C(O)C6)=CC(O)=CC5

Synonyms:

• (2,5-Dioxopyrrolidin-1-yl) 3′,6′-dihydroxy-1-oxospiro[2-benzofuran-3,9′-xanthene]-5-carboxylate
• 1-{[(3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-6-yl)carbonyl]oxy}pyrrolidine-2,5-dione
• 2,5-Pyrrolidinedione, 1-[[(3′,6′-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-6-yl)carbonyl]oxy]-
• 4-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid
• 6-Carboxyfluorescein Se
• 6-Carboxyfluorescein Succinimide Ester
• 6-Carboxyfluorescein, Succinimidyl Ester
• 6-Carboxyfluorescein-N-Hydroxysuccinimide Ester
• 6-Carboxyfluorescein-Nhs
• 6-Fam, Se
• 6-Fam-Nhs
• 6-carboxyfluorescein, succinimidyl ester (6-FAM, SE)
• Fluorescein-6-carboxylic acid N-succinimidyl ester
• Spiro[isobenzofuran-1(3H),9′-[9H]xanthene], 2,5-pyrrolidinedione deriv.

2,5-Pyrrolidinedione,1-[[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-6-yl)carbonyl]oxy]-

CAS: 92557-81-8

Formula: C₂₅H₁₅NO₉

Purity: 90%

Color and Shape: Solid

Molecular weight: 473.3879

Ref: IN-DA00IKKZ

6-FAM SE

CAS: 92557-81-8

6-FAM SE

Purity: ≥98%

Molecular weight: 473.39g/mol

Ref: 54-BUP11104

6-Carboxyfluorescein N-hydroxy succinimide ester

CAS: 92557-81-8

6-Carboxyfluorescein N-hydroxy succinimide ester

Purity: 97%

Color and Shape: Solid

Molecular weight: 473.39g/mol

Ref: 54-BIC1055

6-Carboxyfluorescein N-hydroxysuccinimide ester

CAS: 92557-81-8

Formula: C₂₅H₁₅NO₉

Molecular weight: 473.39

Ref: 7W-GT3778

6-FAM SE

CAS: 92557-81-8

6-FAM SE (6-Carboxyfluorescein N-succinimidyl ester) is another isomer of carboxyfluorescein.

Formula: C₂₅H₁₅NO₉

Purity: 98.23%

Color and Shape: Solid

Molecular weight: 473.39

Ref: TM-T18901

6-Carboxyfluorescein-N-hydroxysuccinimide ester

CAS: 92557-81-8

6-Carboxyfluorescein is a fluorescent dye widely used in PCR methodologies. It is used to label the 5’ end of oligonucleotides as a reporter in the presence of a quencher at the 3’ end (dual labelled probe). During amplification, the dye is cleaved, and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. It is used to tag DNA and RNA for in vivo and in vitro applications, as well as for structural studies and RNA-protein interactions. 6-Carboxyfluorescein that has NHS-activated carboxylic acids can react with primary amines. 5’-reporter used in RT-PCR for COVID-19 diagnosis by HKI; Institute Pasteur; Charité; CDC, USA; CDC, China; NIID and NIH. (ex/em: 495/517nm in DMSO or DMF at pH > 9.0)

Formula: C₂₅H₁₅NO₉

Purity: Min. 95%

Color and Shape: Yellow Powder

Molecular weight: 473.39 g/mol

Ref: 3D-FC19778