Z-Phe-Cit-AMC, with the CAS number 92745-52-3, is a synthetic compound often used in biochemical research, particularly in the study of proteases and enzyme activity. It is a peptide substrate that consists of a phenylalanine (Phe) residue linked to a citrulline (Cit) moiety and an aminomethylcoumarin (AMC) fluorophore. The structure of Z-Phe-Cit-AMC allows it to be cleaved by specific proteases, releasing the AMC group, which results in a measurable fluorescence signal. This property makes it valuable for monitoring enzyme activity in various assays. The compound is typically characterized by its stability under physiological conditions and its ability to provide a sensitive and quantitative measure of proteolytic activity. Additionally, Z-Phe-Cit-AMC is often utilized in drug discovery and development, as it helps in screening potential inhibitors of proteases, contributing to the understanding of various biological processes and disease mechanisms.

Z-Phe-Cit-AMC

92745-52-3: Z-Phe-Cit-AMC, with the CAS number 92745-52-3, is a synthetic compound often used in biochemical research, particularly in the study of proteases and enzyme activity. It is a peptide substrate that consists of a phenylalanine (Phe) residue linked to a citrulline (Cit) moiety and an aminomethylcoumarin (AMC) fluorophore. The structure of Z-Phe-Cit-AMC allows it to be cleaved by specific proteases, releasing the AMC group, which results in a measurable fluorescence signal. This property makes it valuable for monitoring enzyme activity in various assays. The compound is typically characterized by its stability under physiological conditions and its ability to provide a sensitive and quantitative measure of proteolytic activity. Additionally, Z-Phe-Cit-AMC is often utilized in drug discovery and development, as it helps in screening potential inhibitors of proteases, contributing to the understanding of various biological processes and disease mechanisms.

<p>Z-Phe-Cit-AMC is a fluorescent substrate for the enzymes cysteine and peptide aminopeptidases. It has been synthesized by conjugating 7-amino-4-methylcoumarin with L-phenylalanine. The product is useful in assays to measure the activity of these enzymes, which are involved in protein digestion and wound healing. Z-Phe-Cit-AMC can be hydrolyzed by bromelain, ficin, or papain and it can be used as a substrate for enzyme reactions.</p>

CAS 92745-52-3

Z-Phe-Cit-AMC

Z-Phe-Cit-AMC

Ref: 3D-FP110564