CAS 97792-39-7

Z-Gly-Gly-Leu-AMC

Description:

Z-Gly-Gly-Leu-AMC, also known by its CAS number 97792-39-7, is a synthetic peptide substrate commonly used in biochemical research, particularly in studies involving proteases. This compound consists of a sequence of amino acids, specifically a Z (benzyloxycarbonyl) protecting group attached to glycine (Gly), glycine (Gly), and leucine (Leu), followed by an AMC (7-amino-4-methylcoumarin) moiety. The Z group serves to protect the amino group of the first glycine, enhancing the stability and specificity of the peptide during enzymatic reactions. Z-Gly-Gly-Leu-AMC is often employed in fluorescence-based assays to monitor proteolytic activity, as the cleavage of the peptide bond releases the fluorescent AMC, allowing for quantification. The compound is soluble in organic solvents and water, making it versatile for various experimental conditions. Its characteristics make it a valuable tool in the study of enzyme kinetics and the development of protease inhibitors.

Formula: C₂₈H₃₂N₄O₇

InChI: InChI=1/C28H32N4O7/c1-4-17(2)26(27(36)31-20-10-11-21-18(3)12-25(35)39-22(21)13-20)32-24(34)15-29-23(33)14-30-28(37)38-16-19-8-6-5-7-9-19/h5-13,17,26H,4,14-16H2,1-3H3,(H,29,33)(H,30,37)(H,31,36)(H,32,34)

SMILES: CCC(C)C(C(=Nc1ccc2c(C)cc(=O)oc2c1)O)N=C(CN=C(CN=C(O)OCc1ccccc1)O)O

Synonyms:

• 7-(N-Benzyloxycarbonylglycyl-glycyl-leucyl)amino-4-methylcoumarin
• 7-Z-Gly-gly-leu-ME
• N-[(benzyloxy)carbonyl]glycylglycyl-N-(4-methyl-2-oxo-2H-chromen-7-yl)isoleucinamide

Z-Gly-Gly-Leu-AMC

CAS: 97792-39-7

Z-GGL-AMC, a fluorogenic substrate for the chymotrypsin-like activity of the 20S proteasome. Sensitive fluorogenic substrate for subtilisins BPN’ and Carlsberg. Also cleaved by ClpP protease from M. tuberculosis.

Formula: C₂₈H₃₂N₄O₇

Purity: >99%

Color and Shape: White Powder

Molecular weight: 536.59

Ref: 01-4013102

Z-Gly-Gly-Leu-AMC

CAS: 97792-39-7

Z-Gly-Gly-Leu-AMC is a small molecule that inhibits the activity of certain enzymes, such as poly (ADP-ribose) polymerase (PARP), which are involved in DNA repair and DNA replication. It has been shown to be effective against cancer cells and is used for the treatment of various cancers, including breast cancer. Z-Gly-Gly-Leu-AMC has also been shown to have anti-inflammatory properties and may be useful for the treatment of autoimmune diseases. The drug binds to PARP, inhibiting its ability to interact with other proteins, thereby preventing activation of proapoptotic protein.

Formula: C₂₈H₃₂N₄O₇

Purity: Min. 95%

Molecular weight: 536.58 g/mol

Ref: 3D-FG110537