CAS 98640-71-2

Collagenase Chromophore Substrate Test Substance (for quantitative Collagenase-Determination)

Description:

Collagenase Chromophore Substrate Test Substance, identified by the CAS number 98640-71-2, is a synthetic substrate designed for the quantitative determination of collagenase activity. This compound typically features a chromophore that undergoes a measurable change upon enzymatic cleavage by collagenase, allowing for the quantification of enzyme activity. The substrate is often used in biochemical assays to evaluate collagenase levels in various biological samples, which can be crucial for research in fields such as tissue remodeling, wound healing, and certain pathological conditions. The characteristics of this substance include its specificity for collagenase, stability under assay conditions, and the ability to produce a distinct colorimetric or fluorometric signal upon reaction. The use of this substrate facilitates the assessment of collagenase activity in both clinical and research settings, contributing to our understanding of collagen metabolism and its implications in health and disease. Proper handling and storage conditions are essential to maintain the integrity of the substrate and ensure accurate assay results.

Formula: C₂₅H₃₀N₄O₅

Synonyms:

• Collagenase-ChroMophore-Substrate Test Substance
• (2S)-4-methyl-2-[[(2S)-1-[(4-phenyldiazenylphenyl)methoxycarbonyl]pyrrolidine-2-carbonyl]amino]pentanoic acid
• 4-phenylazo-Z-L-prolyl-L-leucine
• L-Leucine, N-[1-[[[4-(phenylazo)phenyl]methoxy]carbonyl]-L-prolyl]- (9CI)
• Collagenase Chromophore Substrate Test Substance (for quantitative Collagenase-Determination)

Pz-Pro-Leu-OH

CAS: 98640-71-2

Cleavage product of Pz-peptide, a substrate for collagenase and thimet oligopeptidase.

Formula: C₂₅H₃₀N₄O₅

Purity: > 99%

Color and Shape: Orange Powder

Molecular weight: 466.54

Ref: 01-4010074

Collagenase-Chromophore-Substrate Test Substance

CAS: 98640-71-2

Formula: C₂₅H₃₀N₄O₅

Purity: ≥ 95.0%

Color and Shape: Orange solid

Molecular weight: 466.53

Ref: 7W-GW5573

Pz-Pro-Leu-OH

CAS: 98640-71-2

Pz-Pro-Leu-OH is a proteolytic enzyme that cleaves proteins by hydrolysis at the peptide bond. It has been shown to be active against Clostridium and has been used in colorimetric methods for the measurement of this bacterium. Pz-Pro-Leu-OH is produced by subtilisin, which is expressed in Escherichia coli. The amount of this enzyme can be monitored and optimized by cloning. Thermolysin, another protease, also cleaves proteins at the peptide bond and has been used to measure the activity of Pz-Pro-Leu-OH.

Formula: C₂₅H₃₀N₄O₅

Purity: Min. 95%

Molecular weight: 466.53 g/mol

Ref: 3D-FP108150