Enzymes in Recombinant Proteins

<p>USP46 Protein, Human, Mouse, Recombinant (SUMO) is expressed in Baculovirus insect cells with SUMO tag.</p>

<p>Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal</p>

<p>D-Amino-Acid Oxidase (DAO) belongs to the DAMOX/DASOX family.</p>

<p>The combination of silencing ENO2 and 2-deoxyglucose (2-DG) synergistically inhibited leukemia cell survival.</p>

<p>Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to</p>

<p>PCK2 promotes tumor initiation by lowering acetyl-CoA level through reducing the mitochondrial tricarboxylic acid (TCA) cycle.</p>

<p>DNMT2, also known as tRNA (cytosine-5-)-methyltransferase, DNA methyltransferase homolog HsaIIP, and TRDMT1, is a member of the DNA methyltransferase family of</p>

<p>β-1,4-galactosyltransferase 4 (B4GALT4) is a single-pass type II membrane protein that belongs to the Glycosyltransferase 7 family.</p>

<p>Guanylyl cyclase C (GUCY2C) has canonical centrality in defense of key intestinal homeostatic mechanisms, encompassing fluid and electrolyte balance, epithelial</p>

<p>ITK Protein, Mouse, Recombinant (aa 351-619, His &amp; GST) is expressed in Baculovirus insect cells with His and GST tag.</p>

<p>Polypeptide N-acetylgalactosaminyltransferase 3(GALNT3) belongs to the glycosyltransferase 2 family and galNAc-T subfamily.</p>

<p>Enterokinase (EK) is a serine protease produced in the duodenum and involved in mammalian digestion. It activates trypsinogen to trypsin, thereby indirectly initiating the activation of pancreatic digestive enzymes. EK specifically cleaves after the recognition sequence Asp-Asp-Asp-Asp-Lys, but is inactive if a proline follows the cleavage site.<br>This recombinant bovine enterokinase is the light chain catalytic subunit, comprising a single glycosylated polypeptide chain corresponding to residues Ile801–His1035 of the native protein (Accession # P98072). Expressed in Pichia pastoris under animal-free conditions, the enzyme is highly active and suitable for applications in drug and vaccine development. A C-terminal 6×His tag is included to allow efficient removal by Ni²⁺ affinity chromatography following cleavage reactions.</p>

<p>SUMO protease, also known as Ulp, is a highly specific enzyme that removes small ubiquitin-like modifier (SUMO) from recombinant SUMO fusion proteins. Unlike proteases such as enterokinase or TEV, which recognize linear amino acid sequences, SUMO protease recognizes the native tertiary structure of SUMO, allowing precise cleavage without leaving extra residues at the cleavage site.<br><br>This recombinant SUMO protease is derived from Saccharomyces cerevisiae, expressed in E. coli, and purified to obtain high yields of active enzyme under animal-free conditions. It is well suited for applications in drug and vaccine development, protein manufacturing, and other research uses. A C-terminal 6×His tag is included to enable easy removal of the protease following cleavage via Ni²⁺ affinity purification.</p>

<p>Fumarase is an enzyme that catalyze the reversible hydration/dehydration of fumarate to S-malate and is involved in the tricarboxylic acid or Krebs cycle.</p>

<p>Enterokinase (EK) is a duodenal protease involved in digestion by activating trypsinogen into trypsin, which in turn activates other pancreatic enzymes. EK specifically cleaves after the sequence Asp-Asp-Asp-Asp-Lys (DDDDK↓) and is inactive if a proline follows the site.<br>Recombinant bovine enterokinase (rbEK) shows higher activity than EK from other species and is widely used in biochemical applications. It is a glycosylated single-chain protein of ~200 amino acids, tagged with C-terminal 6×His for Ni²⁺ affinity purification and easy removal after digestion. The purified rbEK has a molecular weight of ~40 kDa and is fully biologically active.</p>

<p>IL-1R1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.</p>

<p>PPIL2, is an enzyme that belongs to the cyclophilin family.</p>

<p>DOPA Decarboxylase/DDC Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag.</p>

<p>ALDH1A3 Protein, Human, Recombinant (His) is expressed in E.</p>

<p>Butyrylcholinesterase Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>