Others

Chromogenic beta-Glucosidase substrate yielding a yellow solution upon cleavage.

5-Bromo-4-chloro-3-indoxyl choline phosphate is a synthetic enzyme substrate for detecting phosphatidylcholine-specific phospholipase C.

Alternative chromogenic substrate for alpha-D-galactosidase

4-Nitrophenyl 3-O-(beta-D-glucopyranosyl)-alpha-D-glucopyranoside is a chromogenic substrate for alpha-glucosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 3-O-(beta-D-glucopyranosyl)-alpha-D-glucopyranoside is used in different applications such as the Gaucher disease screening, carbohydrate metabolism studies

Espresso-Gal (Espresso-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields an espresso brown colored precipitate. Espresso-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.

4-Nitrophenyl phosphate bis(cyclohexylammonium) is an excellent chromogenic substrate designed for the detection and quantification of phosphatase enzyme activities. Aided by the presence of the cyclohexylammonium cation, it undergoes enzymatic hydrolysis, yielding the colored 4-nitrophenolate ion, which can be readily observed at 405 nm. The substrate has become an essential tool for biomedical research, playing a crucial role in unearthing cellular mechanisms, function, and potential drug targets.

A chromogenic substrate for β-galactosidase.

4-Fluoro-2-nitrophenyl beta-D-galactopyranoside, a fluorinated analog of ONPG, is a chromogenic enzyme substrate specifically used to detect beta-galactosidase in bacterial media. 4-Fluoro-2-nitrophenyl beta-D-galactopyranoside is used in Lactose intolerance screening, bacterial beta-galactosidase detection (E. coli)

5-Iodo-3-indolyl b-D-galactopyranoside is a chromogenic substrate used for beta-galactosidase. It is incorporated into media or detection kits for rapid and direct identification of microorganisms.

Naphthol AS-D is a diagnostic chemical for phosphatase enzymes and is used to stain cells, tissues, and body fluids for diagnosis. The chemical reacts with the amino groups of proteins in cells and produces a brown color. Naphthol AS-D is not toxic to living cells in concentrations below 5%. It stains basophilic leukemia cells as well as brain cells. This chemical can be used to diagnose cutaneous lesions, such as psoriasis or melanoma, which are difficult to diagnose by standard methods.  present in these lesions and produce a brown color.

Chromogenic substrate for beta-glucosidase

Chromogenic substrate targeting cellulases

4-Nitrophenyl beta-D-glucopyranoside-6-phosphate is a chromogenic substrate for glucose-6-phosphatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-D-glucopyranoside-6-phosphate is used in different applications such as the Glycogen storage disease diagnosis (e.g., von Gierke disease)

6-Chloro-3-indolyl phosphate disodium salt is a chromogenic substrate that is used in food testing, diagnostics, and environmental testing. It can be used to detect the presence of the enzyme phosphatase by adding it to a sample containing glucose, which will produce an orange color. The ligand 6-chloro-3-indolyl phosphate disodium salt can also be used as a bioluminescence probe for detecting ATP or ADP using luciferase. This product has high purity and quality, and is available at a competitive price.

Substrate for feruloyl esterases

Aldol® 518 myo-inositol phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13) activity. The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms grown in liquid or solid media, yielding intense red color. In combination with Aldol® 355 fluorescence enhancer, red fluorescence can also be obtained. Aldol® 518 myo-inositol phosphate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus species. Aldol® 518 myo-inositol phosphate is suitable for use under aerobic and anaerobic conditions.

6-Chloro-3-indoxyl palmitate is a high quality, pure chemical reagent that can be used as a substrate for enzyme activity. It is a chromogenic substrate that is used in the detection of esterase activity. 6-Chloro-3-indoxyl palmitate is also used as a fluorogenic substrate in the presence of horseradish peroxidase and hydrogen peroxide to produce chemiluminescence. 6-Chloro-3-indoxyl palmitate has been shown to be an effective bioluminescent substrate for luciferase enzymes, such as firefly luciferase and Renilla luciferase.