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Aldol® 470 L-pyroglutamic acid amide is a chromogenic and fluorogenic enzyme substrate used for detection of pyrrolidonyl peptidase (PYRase) activity. The colorless enzyme substrate is cleaved by PYRase-producing bacteria grown in liquid or solid media, yielding orange color. In addition, green fluorescence is generated when a suitable matrix is present, such as, the surface of cellulose acetate filters, polypropylene tubes or microplates, and polyethylene vials. Aldol® 470 L-pyroglutamic acid amide is suitable for use under aerobic and anaerobic conditions.

4-Nitrophenyl 2,6-di-O-benzoyl-a-D-galactopyranoside is a specialized chromogenic substrate for the accurate evaluation of α-galactosidase activity. Upon enzymatic hydrolysis, it generates the chromophore 4-nitrophenol, which can be conveniently quantified by absorbance spectroscopy. The substrate features a unique benzoylated galactopyranoside moiety for enhanced selectivity and compound stability. This high-quality substrate is perfect for various research, analytical, and diagnostic applications involving α-galactosidase detection or quantification.

Jalapeno green-Gal (Jalapeno green-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a Jalapeno green colored precipitate. Jalapeno green-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach.

4-Nitrophenyl 2,4,6-tri-O-acetyl-3-O (2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-a-D-glucopyranoside is a chemiluminescent substrate that can be used for the detection of aminoglycosides in biological fluids. It is a high purity and high quality product with CAS No. 482743. The product has been tested for safety and purity using culture media and environmental testing. This product may be used as a bioluminescence substrate to detect the presence of aminoglycosides in samples such as urine, serum or plasma. 4NTPAGDG is also useful as a fluorogenic substrate for diagnostics and enzyme substrates for food testing.

Naphthol AS-BI b-D-glucopyranoside is a substrate for enzymes such as β-glucosidase and β-glycosidase. It has been used in diagnostic tests to detect the presence of amylase, lipase, α-amylase, or maltase in urine and feces. In biochemistry, naphthol AS-BI b-D-glucopyranoside is often used as a chromogenic or fluorogenic substrate for the detection of β-galactosidases and other glycosidases. This product has also been used in the detection of bacteria in culture media. Naphthol AS-BI b-D-glucopyranoside is a high quality reagent that can be used for chemiluminescence experiments, food testing, and environmental testing.

4-Nitrophenyl 6-O-DMT-2,3,4-tri-O-levulinoyl-a-D-mannopyranoside is a fluorogenic substrate that can be used for the detection of DMT (dihydroxyacetone) in food products. It has been shown to be an excellent alternative to the more commonly used 4,5′,6,7,8,-Tetrabromoindoxyl 3′,5′,6′,-tetraacetic acid (TBA). This product is also used as a chromogenic substrate for the determination of phenols in environmental testing and as a ligand conjugate for immunohistochemistry staining. The purity of this product is guaranteed and it has been tested extensively.

4-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-a-D-glucopyranoside is a synthetic compound with the ability to inhibit the enzyme glutamicum. It is a radionuclide that can be used as a focusing agent for gamma cameras and has been shown to be effective against corynebacterium glutamicum and other organisms. 4NP2AG was synthesized by reacting nitrophenol with acetamide in the presence of an acid catalyst and hydrogenating the product. The synthesis employs precession chemistry, which involves rotating a molecule around its axis at high speed to increase its concentration in one area. This treatment leads to a higher yield of desired product. 4NP2AG contains two acetamido groups on the glucopyranoside moiety, which are esterified with nitrophenol and acetic acid respectively. These moieties are

4-Nitrophenyl 2-O-(α-D-glucopyranosyl)-α-D-glucopyranoside is a specialized chromogenic pNP substrate featuring dual glucose-linked moieties, designed for accurate detection and quantification of specific glycosidase enzymes. Upon cleavage by target enzymes, this substrate releases 4-Nitrophenol, which can be easily monitored spectrophotometrically as a yellow color change. This advanced substrate offers enhanced specificity and sensitivity in enzyme assays, facilitating cutting-edge research in biochemistry, enzymology, and other related fields.

Aldol® 467 N-acetyl-beta-D-glucosaminide is a chromogenic and fluorogenic enzyme substrate used for detection of beta-N-acetyl-glucosaminidase activity. The colorless enzyme substrate is cleaved in the presence of beta-N-acetyl-glucosaminidase yielding development of a yellow product. In addition, green fluorescence is generated when a suitable matrix or Aldol® 355 fluorescence enhancer is present. Aldol® 467 N-acetyl-beta-D-glucosaminide amide can be used in enzymatic assays or in live bacterial cultures. Aldol® 467 N-acetyl-beta-D-glucosaminide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

5-Bromo-3-indoxyl-beta-D-galactopyranoside (5BIG) is a fluorogenic substrate that has been shown to be an effective enzyme substrate. It is used in the chemiluminescence, bioluminescence, and fluorescence detection of enzymes. 5BIG is also a chromogenic substrate for nucleic acid staining and can be used in food testing as well as diagnostics. 5BIG is highly pure and high quality with a CAS number of 1301706-59-1.

4-Nitrophenyl b-D-thiomannopyranoside acts as a substrate for β-mannosidase and thioglycoligase. Upon enzymatic cleavage, these substrates release 4-nitrophenol, a yellow chromophore that can be detected spectrophotometrically at 405 nm, facilitating quantitative measurements of enzymatic activity. Consequently, these substrates are widely used in biochemical research, clinical diagnostics, and drug discovery for evaluating the function of specific enzymes in various biological processes.

5-Bromo-4-chloro-3-indoxyl-alpha-D-xylopyranoside is an enzyme substrate that can be used in the detection of β-galactosidase activity. This product has been shown to be a good ligand for various xanthine oxidoreductases, including xanthine dehydrogenase and uricase. 5BBIX is also a chromogenic substrate for horseradish peroxidase and can be used to detect hydrogen peroxide in the presence of osmium tetroxide or potassium permanganate. 5BBIX has been extensively tested for use in diagnostics, food, and environmental testing.

4-Nitrophenyl beta-galactoside (pNP-Gal) is a chromogenic substrate commonly used in enzymatic assays to detect and quantify the presence of beta-galactosidase. It is hydrolyzed by the enzyme to release 4-nitrophenol, which produces a yellow color that can be measured spectrophotometrically. Similarly, 4-Nitrophenyl beta-glucoside (pNP-Glc) can be used to assay beta-glucosidase activity. 4-Nitrophenyl beta-globotrioside (pNP-Glo) is a similar compound that has been designed as a substrate for glycosidases that cleave terminal beta-galactosyl-(1->3)-N-acetylgalactosamine residues. In these assays, the release of 4-nitrophenol can be measured to determine the activity of the enzyme under study. The use of chromogenic substrates like pNP-Glo allows for the rapid and reliable detection of glycosidase activity in a variety of biological samples.

1-Naphthyl-alpha-D-mannopyranoside is a chromogenic substrate. It is used in diagnostics and as a reagent for enzymatic analysis of alpha-mannosidase activity in culture media, food testing, and environmental testing.

Modified naphtol AS substrate used in histochemical and cytochemical staining

4-Nitrophenyl alpha-D-maltotetraoside is a chromogenic substrate used for the detection of alpha-glucosidase and alpha-amylase activity. The four glucose units in the molecule are hydrolyzed by the enzyme, releasing 4-nitrophenol and allowing for easy colorimetric measurement of enzymatic activity

4-Nitrophenyl-myo-inositol-1-yl phosphate is a highly specific chromogenic substrate used for the detection, quantification, and characterization of phosphoinositide phosphatases. When cleaved by the target enzyme, it generates a highly absorbing 4-nitrophenyl moiety, producing a yellow color that can be measured spectrophotometrically. This versatile substrate is valuable in examining phosphoinositide phosphatase activity, enzyme kinetics, substrate specificity, and inhibition studies in various cellular processes, including signal transduction, lipid metabolism, and membrane trafficking.