Enzymes

EcoRI is an endonuclease enzyme isolated from strains of Escherichia. Coli RY13, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is GAATTC, which, as the complementary sequence is CTTAAG, has rotational symmetry.

<p>BamHI is an endonuclease enzyme isolated from strains of B.amyloliquefaciens H, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is GAATCC, which, as the complementary sequence is CTTAGG, has rotational symmetry.</p>

KpnI is an endonuclease enzyme isolated from strains of Klebsiella pneumoniae Ok8, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is GGTACC, which, as the complementary sequence is CCATGG, has rotational symmetry.

<p>NotI is an endonuclease enzyme isolated from strains of Nocardia otitidis-caviarum and is part of the restriction modification system.In molecular biology it is used as a restriction enzyme. It creates sticky ends with a 3' overhang. The nucleic acid sequence where the enzyme cuts is GCGGCCGC, which, as the complementary sequence is CGCCGGCG, has rotational symmetry. </p>

EcoRV is an endonuclease enzyme isolated from strains of Escherichia.coli J62plg 74, and is part of the restriction modification system. It is also known as Eco321. In molecular biology it is used as a restriction enzyme. It creates blunt ends The nucleic acid sequence where the enzyme cuts is GATATC, which, as the complementary sequence is CTATAG, has rotational symmetry.

<p>XbaI is an endonuclease enzyme isolated from strains of Xanthomonas badrii, and is part of the restriction modification system.<br> In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is TCTAGA, which, as the complementary sequence is AGATCT, has rotational symmetry. </p>

BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn SalI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity SalI binds at the recognition sequence GTCGAC and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm but Inhibition by CpG methylation. It is derived from the E.coli strain encoding SalI gene from Streptomyces albus G

BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn NotI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity NotI binds at the recognition sequence GCGGCCGC and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm methylation but Inhibition by CpG methylation. It is derived from the E.coli strain encoding NotI gene from Nocardia otitidis-caviarum

BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn NdeI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity NdeI binds at the recognition sequence CATATG and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm or CpG methylation. It is derived from the E.coli strain encoding NdeI gene from Neisseria denitrificans

<p>BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn KpnI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity KpnI binds at the recognition sequence GGTACC and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm or CpG methylation. It is derived from the E.coli strain encoding KpnI gene from Klebsiella pneumoniae OK8</p>

<p>BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn BamHI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity BamHI binds at the recognition sequence GGATCC and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm or CpG methylation. It is derived from the E.coli strain encoding BamHI gene from Bacillus amyloliquefaciens H</p>

<p>BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn XhoI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity XhoI binds at the recognition sequence CTCGAG and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm methylation but Inhibition by CpG methylation. It is derived from the E.coli strain encoding XhoI gene from Xanthomonas holcicola</p>

<p>BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn PstI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity PstI binds at the recognition sequence CTGCAG and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm methylation but Inhibition by CpG methylation. It is derived from the E.coli strain encoding PstI gene from Providencia stuartii</p>

<p>BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn SmaI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity SmaI binds at the recognition sequence CCCGGG and cleaves these sequences. It creates blunt ends. Not sensitive to dam, dcm but Inhibition by CpG methylation. It is derived from the E.coli strain encoding SmaI gene from Serratia marcescens</p>

BioLit QuickRxn Restriction Enzyme is highly purified and quickly digests the substrate DNA. QuickRxn NcoI is capable of digesting DNA in 15 minutes or less with the flexibility to digest overnight without degradation to DNA. It is accompained with the specific QuickRxn Buffer to enable 100 % enzyme activity NcoI binds at the recognition sequence CCATGG and cleaves these sequences. It creates sticky ends. Not sensitive to dam, dcm or CpG methylation. It is derived from the E.coli strain encoding NcoI gene from Nocardia carolina

<p>This is an Isoschizomers of BshFI<br> Source :Haemophilus aegyptius<br> 5'-GG↓CC-3'<br> 3'-CC↑GG-5' </p>

<p>Isoschizomers: BfuCI, Bsp143I, BstENII, BstMBI, DpnII, Kzo9II, NdeII<br> Neoschizomers: BstKTI<br> Source: Moraxella bovis, recombinant, Streptomyces sp.<br> 5'-↓GATC-3'<br> 3'-CTAG↑-5' </p>

<p>BstEII is an endonuclease enzyme isolated from strains of Bacillus stearothermophilus , and is part of the restriction modification system.<br> In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is GGTNACC, which, as the complementary sequence is CCANTGG, has rotational symmetry. </p>

BglII is an endonuclease enzyme isolated from strains of Bacillus globigii lacking BglII and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme that creates sticky ends. The nucleic acid sequence where the enzyme cuts is AGATCT, which as the complementary sequence is TCTAGA.

<p>AluI is an endonuclease enzyme isolated from strains of Arthrobacter luteus, and is part of the restriction modification system.<br> In molecular biology it is used as a restriction enzyme. It creates blunt ends. The nucleic acid sequence where the enzyme cuts is AGCT which, as the complementary sequence is TCGA, has rotational symmetry. </p>

<p>RsaI is an endonuclease enzyme isolated from strains of E.coli carrying the gene from Rhodopseudomonas sphaeroides and is part of the restriction modification system.<br> In molecular biology it is used as a restriction enzyme. It creates blunt ends. The nucleic acid sequence where the enzyme cuts is GTAC, which, as the complementary sequence is CATG, has rotational symmetry. </p>

XhoI is an Isoschizomer of SlaI. Source: Streptomyces lavendulae 5'-C↓TCGA G-3' 3'-G AGCT↑C-5'

SmaI is an endonuclease enzyme isolated from strains of Serratia marcescens and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates blunt ends . The nucleic acid sequence where the enzyme cuts is CCCGGG, which, as the complementary sequence is GGGCCC.

<p>An Unique buffer that can be used for most Restriction enzyme reaction set-ups. This buffer aides in optimal digestion within the specified time with great efficiency</p>

<p>HinfI is an endonuclease enzyme isolated from strains of Haemophilus influenzae Rf, recombinant, E. coli and is part of the restriction modification system. <br> In molecular biology it is used as a restriction enzyme. It creates sticky ends with a 3&amp;apos; overhang. The nucleic acid sequence where the enzyme cuts is GANTC, which, as the complementary sequence is CTNAG, has rotational symmetry. </p>

<p>QuickRxn HindIII is expressed and purified from E.coli that carries the recombinant HindIII gene. In molecular biology it is used as a restriction enzyme of 35.8kDa. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is AAGCTT, which, as the complementary sequence is TTCGAA, has rotational symmetry. The reaction is conducted for 5 -15 minutes at 37°C( Quick digestion with high fidelity) and heat-inactivated at 65°C for 20 minutes. Not sensitive to dam, dcm or CpG methylation. It is accompanied with the specific QuickRxn Buffer to enable 100 % enzyme activity.Concentration: 20U/mcl </p>

<p>SalI is an endonuclease enzyme isolated from strains of Streptomyces albus G and is part of the restriction modification system.<br> In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is GTCGAC, which, as the complementary sequence is CAGCTG, has rotational symmetry. </p>

<p>SacI is an Isoschizomers of SstI,<br> Neoschizomers: Ecl136II, EcoICRI<br> Source: Streptomyces Stanford<br> 5'-G AGCT↓C-3'<br> 3'-C↑TCGA G-5' </p>