Enzyme Substrates

<p>Sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases.</p>

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<p>Aldol® 484 alpha-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate for alpha-glucosidase activity (EC 3.2.1.20, alpha-1,4-glucosidase, also known as maltase). The colorless enzyme substrate is cleaved by bacteria producing this enzyme in liquid or solid media, yielding orange color. Green fluorescence is also generated if a suitable matrix is present such as: cellulose acetate filters, polypropylene tubes or microplates and polyethylene vials. Aldol® 484 alpha-D-glucopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>4-Methylumbelliferyl beta-D-glucuronide (MUG) is commonly used as a substrate for detecting beta-glucuronidase. MUG is used for identifying E. coli contamination in food, drinking water, clinical samples and for rapid bacterial identification in blood cultures. It is recommended in international normatives (ISO, DIN) as a fluorogenic substrate for detection of E. coli. MUG is also a tool assaying the glucuronidase gene in plants.</p>

<p>Luc-phosphate Na enhanced is a bioluminescent substrate used to detect phosphatase activity in bacterial assays.</p>

<p>5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-glucosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is used in Pompe disease screening and glycogen storage disorder diagnosis.</p>

<p>Water soluble substrate for firefly luciferase.</p>

<p>Bioluminescent substrate for luciferase</p>

<p>Histochemical substrate for esterase</p>

<p>Selenium analogue of firefly luciferin with red-shifted bioluminescence</p>

<p>Luc-Enterobacteria Na is a fluorogenic substrate that can be used for the detection of Enterobacteriaceae. This product has been shown to have high purity and a CAS number. Luc-Enterobacteria Na is also conjugated with an enzyme substrate, making it suitable for detecting the presence of bacterial colonies in culture media.</p>

<p>Reducing co-substrate for heme peroxidases; test reagent in microbiology</p>

<p>Esculetin-7-beta-D-galactopyranoside, also known as 6,7-Dihydroxycoumaryl-7-beta-D-galactopyranoside, is a fluorogenic substrate used in the detection of beta-galactosidase activity. This coumarin derivative, when hydrolyzed, yields esculetin (6,7-dihydroxycoumarin) which, in the presence of iron salts, forms a brown-black complex.</p>

<p>Chromogenic substrate to visualize the activity of alpha-D-mannopyranoside; red color produced</p>

<p>5-Bromo-4-Chloro-3-Indoxyl-N-Acetyl-Beta-D-Galactosaminide is a chromogenic substrate for detecting beta-galactosidase and GalNAc-transferase enzymes. Upon hydrolysis by the enzyme, it produces a blue-green colored compound that can be detected visually or measured spectrophotometrically. This substrate is useful in characterizing the activity of Beta-Galactosidase and GalNAc-transferase enzymes involved in glycobiology research and diagnostic applications.</p>

<p>4-Methylumbelliferyl 3-deoxy-D-glycero-a-D-galacto-2-nonulosonic acid sodium salt is a fluorogenic substrate that can be used to detect enzyme activity. It reacts with the enzyme β-galactosidase, which hydrolyzes lactose in the presence of oxygen and hydrogen peroxide. The product is 4methylumbelliferone, which emits light in the presence of luciferin and an oxidizing agent. This product is used for diagnostics, food testing, and environmental testing. CAS No. 123269-95-4</p>

<p>Chromogenic sulfatase substrate</p>

<p>3-(7-Hydroxycoumarin-3-carboxamido)propyl 4-O-(b-D-galactopyranosyl)-b-D-glucopyranoside is a fluorogenic substrate that is used for the detection of beta-galactosidase. It has been shown to be an efficient substrate for the enzyme beta galactosidase and can be used to detect this enzyme in cell culture media and other diagnostic applications. 3-(7-Hydroxycoumarin-3-carboxamido)propyl 4-O-(b-D-galactopyranosyl)-b-D-glucopyranoside can also be used as a chromogenic substrate, which is added to a reaction mixture containing beta galactosidase, where it is hydrolyzed by the enzyme to produce a blue color. This product also has food testing and environmental testing applications.</p>

<p>4-Nitrophenyl beta-L-arabinopyranoside is a chromogenic substrate for beta-L-arabinosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-L-arabinopyranoside is used in different applications such as the Bacterial arabinan degradation assays, gut microbiome analysis</p>

<p>1-Naphthyl b-D-glucuronide sodium salt is a chromogenic substrate commonly used to assay the activity of beta-glucuronidase enzymes. It is hydrolyzed by beta-glucuronidase to yield a yellow-colored product, 1-naphthol, which can be quantified spectrophotometrically. This substrate is widely used in diagnostic tests for detection of beta-glucuronidase activity, particularly in clinical microbiology for the identification of E. coli and other enteric bacteria. With its sodium salt form, it boasts increased solubility in aqueous solutions, ensuring seamless integration into various experimental settings.</p>