Enzyme Substrates

<p>2-O-(4-Nitrophenyl)-a-D-N-glycolylneuraminic acid is commonly used to measure the activity of sialidase enzymes, specifically those that cleave glycoconjugates containing 2-O-(4-nitrophenyl)-alpha-D-N-glycolylneuraminic acid. The resulting product can be detected by measuring the absorbance at a specific wavelength using a spectrophotometer. This substrate offers a high degree of specificity for glycolylneuraminic acid-containing substrates and is useful for both research and diagnostic purposes.</p>

<p>Synthetic chromogenic substrate for detection of lipase enzyme activity that produces a red coloured product, Suitable for use in serum of plasma matrices. This lipase substrate is commonly used in veterinary diagnostics for monitoring and detecting canine pancreatitis,</p>

<p>5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-L-arabinofuranosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside is used in bacterial arabinan degradation assays (e.g. Bacillus spp.).</p>

<p>Water soluble substrate for firefly luciferase. Used for imaging the expression of the LUC marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. Extensively utilized in reporter gene assays, immunoassays and hygiene monitoring. Endotoxin free product.</p>

<p>4-Methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-alpha-L-fucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-alpha-L-fucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for fucosidosis diagnosis.</p>

<p>Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of α-L-fucosidase, especially in human serum.</p>

<p>Aldol® 455 alpha-D-glucopyranoside is a high quality, environmentally tested, staining, and ligand for bioluminescent assays. It has been shown to be a chromogenic substrate for diagnostics and a fluorogenic substrate for enzymes such as alpha-glucosidase, producing a yellow/green-orange color. Aldol® 455 alpha-D-glucopyranoside is also used in culture media and food testing.</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a high-quality fluorogenic substrate specifically designed for the detection and quantification of various enzymes, including beta-glucosidases and sulfatases. Upon hydrolysis by the target enzyme, this substrate releases the highly fluorescent 4-methylumbelliferone, allowing for real-time monitoring and accurate measurement of enzyme activities in biochemical assays, diagnostic tests, and research applications. 4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt has excellent solubility in water, ensuring a consistent and reliable performance in different experimental conditions. This versatile substrate is an essential tool for researchers and industry professionals who require a sensitive and reproducible detection method for enzymes of interest.</p>

<p>5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, cyclohexylammonium salt is a chemiluminescent substrate that provides a significant increase in light emission. It has a CAS number of 59820-92-0 and is supplied in high purity and high quality. This product is used for the detection of various biological reactions, including diagnostics, enzyme substrates, food testing, and more.</p>

<p>Resorufin beta-D-cellobioside is a fluorogenic substrate for the enzyme beta-glucosidase. It is soluble in water and can be used as a chromogenic substrate for beta-galactosidase. Resorufin beta-D-cellobioside is also used as a ligand to study protein interactions, such as those between lectins and glycoproteins. This product is of high purity and quality, with CAS No. 1000404-48-7.</p>

<p>This botanical is a download for the Floral Database.</p>

<p>4-Nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside is a chromogenic substrate for alpha-L-arabinofuranosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside is used in different applications such as the Plant biomass analysis, bacterial arabinan degradation assays</p>

<p>Substrate for hydrolases</p>

<p>2-Fluoro-4-nitrophenyl b-D-galactopyranoside is a chromogenic substrate in enzymatic assays with β-galactosidase. Commonly abbreviated as PFONPG, it is a hydrophilic compound that can be dissolved in saline and blood samples, which makes it a suitable candidate for diagnostic applications as a gene reporter probe by simple analytic methodologies. 2-Fluoro-4-nitrophenyl b-D-galactopyranoside have been used to detect and monitor the growth of prostate tumor cells.</p>

<p>Ethyl umbelliferone-3-carboxylate-a-D-glucopyranoside is a fluorogenic substrate used for detecting alpha-glucosidase activity in enzymatic assays. When alpha-glucosidase cleaves , the glycosidic bond, umbelliferone-3-carboxylate is released, which exhibits strong fluorescence. This substrate is commonly utilized in glycobiology research, diagnostic tests, and high-throughput screening of enzyme inhibitors.</p>

<p>4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-3-O-(b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside is a specific chromogenic substrate designed for the highly sensitive and accurate detection of glycosidase enzyme activity. Glycosidases are responsible for the cleavage of glycosidic bonds between saccharide units in various carbohydrates. The hydrolysis of this substrate by glycosidase enzymes produces 4-nitrophenol, a yellow-colored compound that can be easily monitored through spectrophotometric measurements due to its strong absorbance at 405 nm. This chromogenic substrate is widely used in the analysis of glycosidase activity in various biological samples, including bacterial, fungal, and plant tissues, as well as in environmental samples and biotechnological applications.</p>

<p>6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.</p>

<p>2-Chloro-4-nitrophenyl b-D-maltoside is a fluorescent substrate used in diagnostic kits. It is used to detect the presence of beta-glucuronidase enzymes, which are found in many bacteria and fungi. 2-Chloro-4-nitrophenyl b-D-maltoside has been shown to be a superior substrate for beta-glucuronidase as it can be used at lower concentrations and is more stable than other substrates. The enzyme hydrolyzes the 2,4 nitrophenyl ester bond and releases the fluorogenic product 4-(2'-hydroxyethyl)benzene diazonium chloride (HED). This product emits light under ultraviolet light, making it ideal for use in Bioluminescence Assays.</p>

<p>4-Methylumbelliferone is a coumarin derivative that inhibits the activity of enzymes such as p-nitrophenyl phosphate oxidase and aminopeptidase. It is used in vitro to study oxidative injury, which plays a role in the pathogenesis of inflammatory diseases, including primary sclerosing cholangitis and bone marrow transplantation. 4-Methylumbelliferone has been shown to induce apoptosis by increasing reactive oxygen species levels and activating toll-like receptor 4 (TLR4). It also has inhibitory effects on osteosarcoma cell proliferation in vitro.</p>

<p>4-Methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranoside is a fluorogenic substrate for beta-glucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranosideas a substrate for measuring the beta-glucosidase activity is used for Gaucher disease diagnosis.</p>